Plasma renin activity (PRA) is often found to increase after myocardial infarction (MI). Elevated PRA may contribute to increased myocardial angiotensin II that is responsible for maladaptive remodeling of the myocardium after MI. We hypothesized that MI would also result in cardiac release of cathepsin D, a ubiquitous lysosomal enzyme with high renin sequence homology. Cathepsin D release from damaged myocardial tissue could contribute to angiotensin formation by acting as an enzymatic alternate to renin. We assessed circulating renin and cathepsin D from both control and MI patient plasma (7-20 hours after MI) using shallow gradient focusing that allowed for independent measurement of both enzymes. Cathepsin D was increased significantly in the plasma after MI (P < 0.001). Furthermore, circulating active cathepsin D metabolites were also significantly elevated after MI (P < 0.04), and contained the majority of cathepsin D activity in plasma. Spiking control plasma with cathepsin D resulted in a variable but significant (P = 0.005) increase in PRA using a clinical assay. We conclude that 7-20 hours after MI, plasma cathepsin D is significantly elevated and most of the active enzymatic activity is circulating as plasma metabolites. Circulating cathepsin D can falsely increase clinical PRA determinations, and may also provide an alternative angiotensin formation pathway after MI.

In the title compound, C15H16N2O6S2, the dihedral angle between the benzene rings is 83.2 (3)°. The mol­ecular conformation is stabilized by an intra­molecular C—H⋯O inter­action. In the crystal structure, mol­ecules are linked by N—H⋯O and C—H⋯O hydrogen bonds and additional stabilization is provided by weak C—H⋯π inter­actions.

Body mass index is the fat content of body whereas hyperuricemia is the condition when serum uric acid level crosses the optimum normal level. Increase in BMI may influence serum uric acid. Objective: To find out the relationship of BMI with serum uric acid level in local population. Study Design: Cross sectional study. Setting: Lady Aitchison hospital Lahore. Period: March 2017- August 2017. Material and Methods: Written informed consent was taken prior to data collection. Detailed history was asked and weight in kilograms and height in meters was noted. Body mass index was calculated. Uric acid was measured by uricase method after taking 1 ml venous blood under aseptic measures. Results: Body mass index and serum uric acid of the subjects were compared with the standard values that were 24.99kg/m2 and 5.7mg/dl and statistical analysis showed a significant difference (p-0.023 and p-0.000) respectively. Simple linear regression revealed 0.391 units change in serum uric acid level with one unit change in BMI. Conclusion: Serum uric acid increased with increase in BMI.

ABSTRACT… Background: Hyperuricemia develops when serum uric acid level exceeds the normal value. Estrogen may influence the level of serum uric acid. Postmenopausal females have a remarkable reduction in its level, so serum estradiol is studied in relation to serum uric acid levels in pre and post-menopausal women. Objective: To find out the relationship of serum Estradiol with serum uric acid level in premenopausal and postmenopausal women in local population. Study Design: Case control study. Setting: This study was conducted in Lady Aitchison Hospital Lahore. Period: March 2017-August 2017. Material and Methods: 134 females were enrolled in total and were grouped in to two. Group A comprised of premenopausal and Group B included postmenopausal females. After complete history and general physical examination, 5 ml venous blood sample under aseptic measures was taken. Serum uric acid was measured by enzymatic and serum estradiol by Enzyme-linked immunosorbent assay method. Results: The mean age of pre and postmenopausal women was 32 and 57 ± 7 years, with significantly lower in premenopause. The mean serum E2 was 91.86 ± 26.71 mg/dL in premenopause and 22.04 ± 9.28 mg/dL in postmenopause, with significantly lower mean in postmenopause. Mean serum uric acid was statistically higher in postmenopause that was 6.04 ± 0.58 mg/dL, when compared to premenopause that was 4.22 ± 0.90 mg/dL. Conclusion: Serum uric acid levels increased due to decreased serum estradiol in postmenopausal women as compared to premenopausal women. Article Citation: Naseem R, Al-Fareed Zafar SM, Jawed S, Mukhtar S, Ijaz F, Aftab RK. Influence of serum estradiol on serum uric acid level in pre and postmenopausal women. Professional Med J 2019; 26(9):1587-1591.