The transplacental transmission is the primary route of Neospora caninum infection in bovine herds around the world. This study aimed to determine the frequency of transplacental transmission of the parasite in dairy cattle of Agreste region of Pernambuco through serological tests (IFAT and ELISA). Three hundred sixteen serum samples from cows and heifers and their offspring were analyzed. The transplacental transmission rate was 72.22% (13/18) for cows and 69.23% (9/13) for heifers by IFAT. ELISA test showed transplacental transmission rate of 43.58% (17/39) for cows and 50% (9/18) for heifers. The transplacental transmission rates were similar, in both groups in test, but a higher seropositivity was found in cows by IFAT. Data were statistically analyzed using the chi-square and Fisher's exact test. A significant relationship of dependence between seropositivity of mothers and their offspring was found. The more frequent IFAT antibody titers and ELISA levels for N. caninum were, respectively, 200 and between four (cows) and five (heifers and offspring). In the Spearman correlation, no association was found between the magnitude of antibody titers for N. caninum between mothers and their offspring. The kappa test showed an index of 0.35, indicating a mild correlation between the serological tests used. The study suggests that cows and heifers are the main transmitters of N. caninum in the studied region and that vertical transmission is the major form of transmission in dairy herds of the Agreste region of Pernambuco.Keywords: Neosporosis, dairy cows, IFAT, ELISA, Northeastern Brazil. ResumoA transmissão transplacentária é a principal via de infecção do Neospora caninum nos rebanhos bovinos em todo o mundo. O presente estudo teve como objetivo determinar a frequência da transmissão transplacentária do parasita em bovinos leiteiros do Agreste Pernambucano, por meio de testes sorológicos (RIFI e ELISA). Foram analisadas 316 amostras de soro de fêmeas bovinas (vacas e novilhas) e de suas crias. A taxa de transmissão transplacentária pela RIFI foi de 72,22% (13/18) para vacas e 69,23% (9/13) para as novilhas. O ELISA teste mostrou taxa de transmissão transplacentária de 43,58% (17/39) para as vacas e 50% (9/18) para as novilhas. As taxas de transmissão transplacentária foram similares para os dois testes em geral, porém uma maior soropositividade foi encontrada nas vacas pela RIFI. Os dados foram estatisticamente analisados pelo teste de qui-quadrado e teste exato de Fischer. Foi encontrada uma relação significativa de dependência entre a soropositividade das mães e de suas crias. Os títulos de anticorpos anti-N. caninum foi de 200 na
ResumoA dilatação do ceco (DC) é um transtorno digestivo de natureza fermentativa com maior ocorrência em vacas de leite, sendo poucos os relatos sobre a sua ocorrência em bezerros. Portanto, este trabalho teve como objetivo relatar as manifestações clínico-cirúrgicas, laboratoriais e anatomopatológicas da DC diagnosticada em quatro bezerros, atendidos na Clínica de Bovinos, Campus Garanhuns da Universidade Federal Rural de Pernambuco (UFRPE). Ao exame clínico dos animais constatou-se um quadro de apatia, desidratação, alteração no apetite, motilidade do rúmen, abomaso e intestinos diminuída e ainda, fezes escassas e diarreicas. Observou-se um aumento na região da fossa paralombar direita e na auscultação, com auxílio da percussão, verificou-se uma ressonância timpânica ("tilintar"). O hemograma revelou uma leucocitose por neutrofilia com desvio para esquerda regenerativo, linfocitose e monocitose, e ainda, uma hiperfibrinogenemia. Na análise do fluido ruminal verificou-se uma elevação do teor de cloretos (>30mEq/L). Foi indicada uma laparotomia exploratória na região do flanco direito, em dois dos bezerros, sendo possível confirmar a DC. Em função da gravidade da lesão e condição clínica precária todos os animais vieram a óbito. Na necropsia, além da DC, foram evidenciadas alterações secundárias a esta enfermidade. Ratifica-se neste trabalho que a DC em bezerros é uma enfermidade de baixa ocorrência, podendo afirmar que o prognóstico é ruim na maioria dos casos, quando diagnosticada tardiamente.Palavras-chave: bovinos, clínica de ruminantes, distúrbio digestivo, intestino grosso, ressonância timpânica. AbstractCecal dilatation is a fermentative disorder of the digestive tract that has a greater occurrence in dairy cows and few reports in calves. Therefore, this study aimed to report the clinical, surgical, laboratorial findings and pathological lesions of the cecal distention in four calves that were diagnosticated and treated at the Clínica de Bovinos, Campus Garanhuns, Universidade Federal Rural de Pernambuco (UFRPE). The clinical findings were apathy, dehydration, lack of appetite, decrease in ruminal, abomasal and intestinal motility and diarrheal and scarce feces. The abdomen was distended on the right side (paralumbar fossa) and there was a tympanic resonance in the same area. The blood assays have shown leukocytosis with neutrophilia with a regenerative left shift, lymphocytosis and monocytosis, and also hyperfibrinogenemia. The ruminal fluid analysis showed a high content of chlorides (>30mEq/L). Exploratory laparotomy on the right flank was performed on two calves that confirmed cecal distention. The severity of the injury and poor clinical condition lead animals to death. At necropsy, in addition to DC, minor changes were observed in this disease. Is ratified this study that DC is a disease in calves of low occurrence and can say that the prognosis is poor in most cases, when diagnosed late.Keywords: bovine, clinical ruminant, digestive disorder, large intestine, tympanic resonance. IntroduçãoA dilata...
In order to differentiate groups of hypocalcemic (G1) and normocalcemic animals (G2) and infer the influence of subclinical hypocalcemia on metabolic profiles, this study determined calcium concentrations during the transitional period in 35 dairy goats healthy, pregnant, primiparous or multiparous, crossbreed or pure-bred dairy goats producing average 3 kg/day/goat. Therefore, blood samples were collected before (30, 20 and 10 days before parturition), on the day of delivery and after parturition (10, 20, 30, 40, 50 and 60 days postpartum). The variables measured were glucose, nonesterified fatty acids, β-hydroxybutyrate, cholesterol, triglycerides, amylase, total protein, albumin, urea, creatinine, aspartate aminotransferase, gamma glutamyl transferase, creatine kinase, total calcium, phosphorus, magnesium, chlorides, cortisol and insulin, as well as ionized calcium (Ca++), sodium and potassium. Goats were considered to have subclinical hypocalcemia if Ca++ ≤0.72 mmol/L. The data were analyzed by ANOVA. In subclinically hypocalcemic goats, serum concentrations of Ca++ decreased earlier (10dbp) than in normocalcemic goats (parturition) and remained lower throughout the transitional period (p=0,004). Among the measured variables, Ca++ showed greater influence on the NEFA, glucose, insulin and total calcium but also influenced the protein profile. Lower food intake by goats with subclinical hypocalcemia was one of the main factors interfering with the metabolic profile and likely the productivity of these animals. Studies should be conducted to measure the effects of subclinical disease on the production rates and on the emergence of other transitional period diseases.
Purpose: Acute mesenteric ischemia (AMI) is a condition in pediatric surgery that ranges from intestine necrosis to death. Ischemic postconditioning (IPoC) methods were developed to reduce the damage caused by revascularization. This study aimed to evaluate the efficacy of these methods in an experimental weaning rat model. Methods: Thirty-two 21-day-old Wistar rats were allocated into four groups according to the surgical procedure performed: control, ischemia-reperfusion injury (IRI), local (LIPoC) and remote IPoC (RIPoC). At euthanasia, fragments of the intestine, liver, lungs, and kidneys were submitted to histological, histomorphometric, and molecular analyses. Results:In the duodenum, intestines, and kidneys histological alterations promoted by IRI were reversed by remote postconditioning method. In the distal ileum, the histomorphometric alterations could be reversed by the postconditioning methods with more evident effects promoted by the remote method. The molecular analysis found that the levels of expression of Bax (proapoptotic) and Bcl-XL (antiapoptotic) genes in the intestine were increased by IRI. These alterations were equally reversed by the postconditioning methods, with more evident effects of the remote method. Conclusion: IPoC methods positively reduced the damage caused by IRI in weaning rats.
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