Inflammation is the protective reaction of body and supporting element of tissues to injurious stimuli, such as physical injury, tissue and cell damage by pathogens, and protein denaturation. This study aims to compare the antiinflammatory activity of ethanol extract, ethanol fraction, and n-hexane fraction from kebiul (Caesalpinia bonduc L.) seed coat. Antiinflammatory activity was tested by measuring the % inhibition of the denatured protein using a UV-Vis spectrophotometer. From the result of the study obtained IC50 value 2,10 μg/mL, 7,89 μg/mL, 4,04 μg/mL & 11,03 μg/mL.
Pemalsuan produk makanan dengan bahan non-halal mulai menjadi masalah baru disekitar masyarakat Indonesia yang mayoritas beragama Islam. Sebagian besar komponen non-halal yang ditemukan di pasar adalah daging babi, lemak babi, gelatin babi dan produk berbasis babi. Pencampuran daging sapi dengan daging babi dalam pembuatan bakso adalah solusi efektif untuk menurunkan harga produksi pembuatan bakso. Proses penelitian ini meliputi proses ekstraksi sokletasi dan kemudian menganalisis kandungan lemak babi menggunakan metode spektroskopi FTIR. Studi penggunaan spektrofotometri Fourier Transform Inframerah (FTIR) dapat digunakan untuk mengidentifikasi adanya daging babi hutan pada bakso sapi secara kualitatif.
Inflammation is a normal protective reaction against tissue damage caused by physical injury, harmful chemicals, and protein denaturation. Protein denaturation is a process in which proteins lose their tertiary structure and secondary structure due to foreign substances, external compounds, such as strong acids, strong bases, organic salts, organic solvents, and heating. The purpose of this study was to determine the anti-inflammatory activity of ethanol extract and ethyl acetate fraction of the seed coat of Kebiul (Caesalpinia bonduc L.) by calculating the IC50 value of protein denaturation in the sample. The results of the phytochemical test of the ethanol extract of Kebiul seeds contained flavonoids, alkaloids, terpenoids, steroids, saponins, and tannins, while the ethyl acetate fraction only contained tannins and alkaloids. The results of the anti-inflammatory test showed that the highest percent inhibition value of sodium diclofenac, ethanol extract, and ethyl acetate fraction, respectively, at a concentration of 20 ppm was 84.8%; 84.1%; and 50%. The IC50 values of sodium diclofenac, ethanol extract, and ethyl acetate fraction were 5.4 μg/mL; 9.9 μg/mL; and 13.3 μg/mL, respectively. The three samples had percent inhibition values exceeding 20% which indicated that all three can be used as an anti-inflammatory.
Keywords: anti-inflammatory, protein denaturation, Caesalpinia bonduc L.
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