Mitochondria play a significant role in beef color. However, the role of oxidative stress in cytochrome c release and mitochondrial degradation is not clear. The objective was to determine the effects of display time on cytochrome c content and oxidation-reduction potential (ORP) of beef longissimus lumborum (LL) and psoas major (PM) muscles. PM discolored by day 3 compared with LL. On day 0, mitochondrial content and mitochondrial oxygen consumption were greater in PM than LL. However, mitochondrial content and oxygen consumption were lower (P < 0.05) in PM than LL by day 7. Conversely, cytochrome c content in sarcoplasm was greater on days 3 and 7 for PM than LL. There were no significant differences in ORP for LL during display, but ORP increased for PM on day 3 when compared with day 0. The results suggest that muscle-specific oxidative stress can affect cytochrome c release and ORP changes.
Interrelationship between mitochondria and myoglobin function influence beef color. NADH level in postmortem muscle is an important determinant of mitochondrial activity and metmyoglobin reduction. Increased aging time promotes discoloration of steaks; however, the mechanism of this effect is not clear. The objective was to characterize the role of wet‐aging in beef longissimus lumborum muscle mitochondrial function and to characterize the global metabolome to determine the mechanism of that can regenerate NADH. Beef longissimus lumborum muscles were randomly assigned to 3, 7, 14, 21, and 28 days aging periods. Surface color, biochemical, mitochondrial, and metabolite profiles were determined at each aging period and at the end of 6‐day display. During 6‐day display, sections aged for 28 days had 30.4% decrease in redness than sections aged for 3 days. Aging time decreased (P <0.05) muscle oxygen consumption, mitochondrial protein content, and antioxidant capacity. Metabolites such as fumaric acid, creatinine, and fructose, that can take part in glycolytic/TCA cycle and regenerate NADH decreased (P <0.05) with aging and display time. In support, NADH levels also decreased (P <0.05) with aging time, but aging time had no effect (P = 0.44) on NADH‐dependent reductase activity. The results suggest that decreased color stability in aged beef can be attributed to increased mitochondrial damage, depletion of metabolites that can regenerate NADH, and increased oxidative stress. Practical Application Beef aging time results in increased discoloration of steaks under retail display. The current research determines the fundamental basis of lower color stability in aged beef. The results indicate that mitochondrial degeneration, depletion of metabolites that produce NADH, and increased oxidative stress can limit shelf‐life of aged steaks. Hence, application of post‐harvest strategies to minimize mitochondrial damage and oxidative changes may have the potential to increase shelf‐life of aged beef.
The objective was to evaluate the effects of wet-aging, rosemary-enhancement, and modified atmospheric packaging on the color of dark-cutting beef during simulated retail display. No-roll dark-cutting strip loins ( = 12; pH > 6.0) were selected from a commercial packing plant within 3 d postharvest. Using a balanced incomplete block design, dark-cutting loins were sectioned in half, and assigned to 1 of 3 aging periods: 7, 14, or 21 d. After respective aging, each aged section was divided into 3 equal parts, and randomly assigned to 1 of 3 enhancement treatments: nonenhanced dark-cutting, dark-cutter enhanced with 0.1% rosemary, and dark-cutter enhanced with 0.2% rosemary. Following enhancement, steaks were randomly assigned to 1 of 3 packaging treatments: high-oxygen modified atmospheric packaging (HiOx-MAP; 80% O and 20% CO), carbon monoxide modified atmospheric packaging (CO-MAP; 0.4% CO, 69.6% N, and 30% CO), and polyvinyl chloride overwrap (PVC; 20% O). Instrumental and visual color measurements were recorded during 5 d simulated retail display. Lipid oxidation was determined utilizing the thiobarbituric acid reactive substances (TBARS) method. There was a significant packaging × enhancement × display time interaction for values and chroma ( 0.001). On d 0 of display, dark-cutting steaks enhanced with 0.1% and 0.2% rosemary and packaged in HiOx-MAP had greater ( 0.001) values and chroma than other dark-cutting packaging/enhancement treatments. A significant packaging × enhancement × display time interaction resulted for values ( 0.001). Dark-cutting steaks enhanced with 0.2% rosemary and packaged in HiOx-MAP was lighter ( 0.001; greater values) than other dark-cutting treatments on d 5 of display. There were no differences ( 0.34) in discoloration scores on d 5 among different dark-cutting treatments when steaks were packaged in HiOx- and CO-MAP. There was an aging period × enhancement × packaging interaction ( < 0.0033) for lipid oxidation. On d 0 of display, there were no differences ( 0.54) in TBARS values between different aging periods and enhancement treatments. Dark-cutting steaks enhanced with 0.2% rosemary had lower ( 0.001) TBARS values than 0.1% rosemary on d 5 when aged for 21 d and in HiOx-MAP. The results suggest that rosemary enhancement with CO- or HiOx-MAP has the potential to improve the surface color of dark-cutting beef.
The objective of this research was to determine the effects of nitrite-embedded/FreshCase packaging on lean color of dark-cutting beef. Eight dark-cutting (pH > 6.0) and eight USDA Low Choice (normal-pH; mean pH = 5.6) beef strip loins (longissimus lumborum) were selected 3 day after harvest. Each dark-cutting loin was sliced into five 2.5-cm thick steaks and randomly assigned to 1) dark-cutting steak packaged in polyvinyl chloride film (PVC) overwrap, 2) dark-cutting steak packaged in nitrite-embedded film, 3) dark-cutting steaks dipped in 0.2% rosemary solution and packaged in nitrite-embedded film, and 4) dark-cutting steak dipped in deionized water and packaged in nitrite-embedded film. The fifth dark-cutting steak was used to determine pH and proximate composition. Normal-pH choice loins were used as a control and each loin was randomly assigned to either PVC overwrap for retail display or to determine pH and proximate composition. Packages were placed in coffin-style retail display cases under continuous fluorescent lighting for 3 days. A HunterLab MiniScan XE Plus spectrophotometer was utilized to characterize steak color every 24 h. There was a significant treatment × storage time interaction (P < 0.05) for a* values and nitric oxide myoglobin formation. On days 1, 2, and 3 of the display, nitrite-embedded treatment improved (P < 0.05) redness compared to other dark-cutting steaks in PVC. A 45% increase in redness (P < 0.05) was observed for nitrite-embedded rosemary treatment over dark-cutting steak in PVC on day 3 of display. Nitric oxide myoglobin formation on day 0 was less for all dark-cutting steaks in nitrite-embedded packaging. Metmyoglobin content was greater (P < 0.05) on day 0 for dark-cutting steaks packaged in nitrite-embedded treatments than dark-cutting steaks in PVC. However, metmyoglobin level in dark-cutting steaks packaged in nitrite-embedded treatments decreased (P < 0.05) on day 1 compared with day 0. Dark-cutting steaks packaged in PVC had greater (P < 0.05) L* values on day 0 than other dark-cutting steaks in nitrite-embedded packaging. Conversely, on days 1, 2, and 3, there were no differences (P > 0.05) in L* values between dark-cutting treatments. Dark-cutting steaks in nitrite-embedded packaging had lower total plate count (P < 0.05) than dark-cutting steak packaged in PVC. The current research indicated that nitrite-embedded packaging has the potential to improve surface color of dark-cutting beef.
Abstract:The objective was to determine the effects of modified atmosphere packaging (MAP) on the surface color of darkcutting beef that had been aged for 21 d. The USDA Choice (normal-pH; IMPS #180) strip loins (n = 10) and no-roll dark-cutter strip loins (n = 10) were obtained from a commercial packing plant within 72 h of harvest. Both normal-pH and dark-cutting beef were vacuum packaged and aged for 21 d. Steaks were cut from both normal and dark-cutting loins, assigned to 1 of 3 packaging treatments; PVC, HiOx-MAP, and CO-MAP, and stored in a simulated retail display under continuous fluorescent lighting at 2°C for 6 d. Instrumental and visual color were measured every 24 h. Thiobarbituric acid assay was used as an indicator for lipid oxidation. There was a packaging × muscle type × display time interaction (P < 0.0001) for instrumental and visual color. On d 1 of display, dark-cutting steaks packaged in HiOx-MAP had greater (P < 0.001) a* values and chroma than darkcutting PVC steaks. On d 6 of the display, dark-cutting steaks packaged in CO-MAP had 10 units greater a* values than darkcutting steaks packaged in PVC. The visual panel also noted less muscle darkening (P < 0.002) in HiOx-MAP and CO-MAP compared with steaks packaged in PVC on d 6 of the display. There was less surface discoloration (P < 0.001) in HiOx-MAP and CO-MAP dark-cutting steaks compared with PVC dark-cutting steaks by the end of the display. There was a packaging × muscle type interaction for instrumental L* values and lipid oxidation. Dark-cutting steaks packaged in HiOx-MAP and CO-MAP had greater (P < 0.05) L* values compared with dark-cutting steaks in PVC packaging. In conclusion, HiOx-MAP improved redness of dark-cutting beef during the initial phase of display, while CO-MAP resulted in a stable red color.
Consumersassociate a bright light pink lean color of ground chicken with freshness andwholesomeness. Chicken meat has greater unsaturated fatty acid composition andlower myoglobin content than beef and pork. As a result, chicken is more prone tooxidative quality changes. Carbon monoxide-modified atmospheric packaging (CO-MAP)creates an anaerobic condition that can limit lipid oxidation and improve color.However, limited knowledge is currently available on ground chicken quality. Therefore,the objective of the current study was to evaluate the color changes and lipidoxidation in ground chicken patties packed under aerobic (PVC), high-oxygen modifiedatmospheric packaging (HiOx; 80% O2+20% CO2), and CO-MAP(CO; 0.4% CO+19.6% CO2+80% N2) and stored at 2 °C. Surfacecolor, lipid oxidation, fatty acid profile, and aerobic plate count were determinedduring storage. Patties packaged in PVC had greater (P< 0.05) pH than HiOx- and CO-MAP. Gas chromatography analysis indicated groundchicken has 72.8% unsaturated fatty acids and 27.2% saturated fatty acids. Theformation of carboxymyoglobin on ground chicken patty surface was confirmed bySoret peak at 420 nm and 570 nm, while oxymyoglobin had peaks at 410 nm and 580nm. Both HiOx- and CO-MAP had greater redness and lower surface discolorationthan PVC on day 4 of storage. Lipid oxidation was greater (P < 0.05) inPVC and HiOx-MAP than CO-MAP. Carbon monoxide inclusion at 0.4% level effectivelyinhibited lipid oxidation and stabilized surface redness during refrigeratedstorage of ground chicken.
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