We carried out a multicenter performance evaluation of three new DNA-based human leukocyte antigen (HLA) typing assays: INNO-LiPA HLA-A Update, INNO-LiPA HLA-B Update, and INNO-LiPA HLA-DQB1 Update. After optimization, the accuracy rates were all 100%, and the final observed resolutions were 99.4, 92.4, and 85.6%, respectively. These rapid and easy-to-perform assays yielded results fully concordant with other DNA-based tissue typing tests.In order to optimize the selection of compatible organ and stem cell donors, DNA-based human leukocyte antigen (HLA) typing assays must not only be accurate but also take into account the continuing discovery of new HLA alleles (2). We recently updated three such HLA tests and then validated the new tests in two phases. First, we performed in-house assessments of the robustness of amplification and of the new probes by using well-characterized samples. In a second phase, reported below, we carried out external performance evaluations of the new assays.The three upgraded tests, INNO-LiPA HLA-A Update, INNO-LiPA HLA-B Update, and INNO-LiPA HLA-DQB1
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