Accurate HLA-A and B allele group and null allele typing using LiPA HLA-A update and LiPA HLA-B update

Canck, I. De; Rombout, A; Thienpont, N; Gielis, M; Sémana, G.; Masson, Dominique; Holman, R.; Casteele, Nancy Vande; Cauter, W Van; Verhelst, A; Vreese, K De; Pughe, Fiona; Barylski, Rachel; Ga, Verpooten

doi:10.1016/s0198-8859(02)00649-3

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“…Identification of new null alleles, or those with altered expression, usually requires a combination of typing by serology and a DNA‐based technique, such as the polymerase chain reaction with sequence‐specific oligonucleotide probes (PCR‐SSOPs) or sequence‐specific primers (PCR‐SSPs). Oligonucleotide primers can then be designed to specifically identify these expression variants (3–6).…”

Section: Hla Class I Types Of A*24020102l (A*24l) Subjects and Their mentioning

confidence: 99%

HLA‐A*24020102L in the UK blood donor population

Dunn¹,

Turton²,

Downing

et al. 2004

Tissue Antigens

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The low-expression allele HLA-A*24020102L was identified on a likely haplotype bearing B*55, Cw*01 during the investigation of nine cases (four families and five unrelated subjects) of HLA-A*24 lacking the A24 specificity. A search was made of 70,007 HLA-A, HLA-B, and HLA-C DNA-based typed largely northwestern European Caucasoid blood donors on the British Bone Marrow Registry and Welsh Bone Marrow Donor Registry panels for phenotypes possessing A*24, B*55, Cw*01. Fifty three were found, and 12 of these were subsequently shown to possess A*24020102L by sequence-based typing or polymerase chain reaction with sequence-specific primers. This indicated that the likely A*24, B*55, Cw*01 haplotype has a frequency of 0.000378 in the UK and that approximately 22.6% of these will possess A*24020102L. Consequently, HLA-A*24020102L, B*55, Cw*01 is a principal A*24020102L-bearing haplotype, and the minimum carriage and gene frequencies of A*24020102L are 0.017% and 0.000086, respectively, in UK blood donors.

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Section: Hla Class I Types Of A*24020102l (A*24l) Subjects and Their mentioning

confidence: 99%