Introduction: Covid-19 pandemic has instigated changes in the teaching and assessment practices of institutions in many parts of the world, resulting not only in online teaching but also in online assessments. The present study analyzes the perceptions of students regarding the first online assessments conducted at a medical college in Pakistan. Materials and Methods: This Mixed method cross-sectional study was conducted in Wah Medical College, National University of Medical Sciences (NUMS), Rawalpindi, Pakistan from July to November 2020. The questionnaire was developed after the collaboration of the authors and a review of the pertinent literature on online examinations. Universal sampling was done and all students of WMC were invited to participate in the study. Data collection was done by google forms and analysis was performed on SPSS. Results: Students from all five years of the MBBS program participated almost equally in the study. Students of 1st, 3rd and 4th year were not contented with their performance in online exams whereas students of 2nd and 5th year were satisfied. Most students considered technological issues as a basis for their poor performance and proposed technical training before online exams. They also provided suggestions related to the improvement of examination formats and tools. Conclusion: The majority of the students found the online assessment more effective and helpful in improving their performance in exams. Student perceptions provided valuable insights for improving the online examinations system at Wah Medical College.
Aim: To study the frequency and factors associated with peritoneal involvement among patients operated for acute appendicitis in a tertiary care hospital. Place and duration of study: Department of Surgery, Benazir Bhutto Hospital Rawalpindi Pakistan from 1stJuly 2020 to 30thJune 2021. Methodology: This comparative cross-sectional study 500 patients diagnosed as acute appendicitis and operated by consultant surgeon were included. Peritoneal involvement was defined as signs of inflammation or infection on abdominal lining observed by operating surgeon during the time of surgery. Factors like age, gender, presence of comorbid illnesses and history of previous abdominal surgeries were associated with presence of peritoneal involvement. Results: There were 305 (61%) males while 195 (39%) were females with mean age was 32.331±4.544 years.Four hundred and forty two (88.4%) did not show any peritoneal involvement at the time of surgery while 58 (11.6%) had peritoneal involvement. Chi-square test revealed that history of previous abdominal surgeries and advancing age had statistically significant association with peritoneal involvement among the study participants (p-value<0.05). Conclusion: Peritoneal involvement was found in considerable number of patients operated as acute appendicitis by the treating surgeons. Patients with previous history of abdominal surgeries and advancing age were more at risk of having peritoneal involvement in our study. Keywords: Acuteappendicitis, Peritonitis, Risk factors, Frequency
Objective: To explore and characterize the microbial fauna associated with the houseflies under different environments.Study Design: Cross-sectional Study.Place and Duration of Study: The study was carried out at the Department of Health Sciences, Capital University of Science and Technology, Islamabad, from July 2022 to December 2022.Materials and Methods: The 50 samples per location were taken from the urban environment of Rawalpindi and Chakwal districts. Then the species of bacteria were isolated for Biochemical and Molecular characters for the most prevalent bacterial species. Antibiotic sensitivity testing was also done for identified species.Results: The biochemical analysis gave significant results regarding the Proteus and Staphylococcus species. Then the phylogenetic analysis of isolated strains showed their close association with Proteus mirabilis in the case of R1_785 and with Proteus Vulgaris and Staphylococcus Xylosus. The identified strains, after the antibiotic sensitivity testing identified to be the most resistant ones moreover, their phylogenetic history showed that they diverged independently as per their evolutionary analysis.Conclusion: The Staphylococcus Xylosus susceptibility was highly resistant against Gentamycin and least resistant against Imipenem and Tazobactam. These findings suggest houseflies' potential role in transmitting pathogenic bacteria with antibiotic resistance in households.
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The gut microbiota of insects is one of the unexplored areas. The association with these microbiomes plays a vital role in supporting their survival and combat with ecological challenges. Mosquito is one of the focal attention insects among the Arthopods, being the vector of many pathogenic diseases including dengue and malaria. A variety of strategies have been designed and implemented to fight against these vectors including obnoxious use of insecticides. Indiscriminate use of insecticides has led to development of resistance against broad range of insecticides. Crucial role of bacteria in insecticide resistance has been under discussion. Many studies focus on the insecticide resistance due to gut microbiome. Thus, the role of gut microbiome is an important area for designing new vector control strategies and their role in improvement of a healthy environment.
Background: Cancers of cervix, head and neck regions have been found to be associated with Human Papilloma Virus (HPV) infection. E1 protein makes an important papillomavirus replication factor. Among the ORFs of papillomaviruses, the most conserved sequence is that of the E1 ORF. It is the viral helicase with being a member of class of ATPases associated with diverse cellular activities (AAA+) helicases. The interactions of E1 with human DNA and proteins occurs in the presence of short linear peptide motifs on E1 identical to those on human proteins. Methods: Different Motifs were identified on HPV16 E1 by using ELMs. Elastic network models were generated by using 3D structures of E1. Their dynamic fluctuations were analyzed on the basis of B factors, correlation analysis and deformation energies. Results: 3 motifs were identified on E1 which can interact with Cdk and Cyclin domains of human proteins. 11 motifs identified on E1 have their CDs of Pkinase on human proteins. LIG_MYND_2 has been identified as involved in stabilizing interaction of E1 with Hsp40 and Hsp70. These motifs and amino acids comprising these motifs play a major role in maintaining interactions with human proteins, ultimately causing infections leading to cancers. Conclusion: Our study identified various motifs on E1 which interact with specific counter domains found in human proteins, already reported having the interactions with E1. We also validated the involvement of these specific motifs containing regions of E1 by modeling elastic networks of E1. These motif involving interactions could be used as drug targets.
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