Subtoxic doses of glyphosate stimulate the growth of a range of plant species, as measured in several plant organs. This hormesis effect is likely to be related to the molecular target of glyphosate, since the effect was not seen in glyphosate-resistant plants, and shikimate levels were enhanced in plants with stimulated growth.

Hybrid (Vitis vinifera ×Vitis labrusca) table grape cultivars grown in the subtropics often fail to accumulate sufficient anthocyanins to achieve good uniform berry color. Growers of V. vinifera table grapes in temperate regions generally use ethephon and, more recently, (S)-cis-abscisic acid (S-ABA) to overcome this problem. The objective of this study was to determine if S-ABA applications at different timings and concentrations have an effect on anthocyanin regulatory and biosynthetic genes, pigment accumulation, and berry color of the Selection 21 cultivar, a new V. vinifera ×V. labrusca hybrid seedless grape that presents lack of red color when grown in subtropical areas. Applications of S-ABA 400 mg/L resulted in a higher accumulation of total anthocyanins and of the individual anthocyaninsanthocyanins: delphinidin-3-glucoside, cyanidin-3-glucoside, peonidin-3-glucoside, and malvidin-3-glucoside in the berry skin and improved the color attributes of the berries. Treatment with two applications at 7 days after véraison (DAV) and 21 DAV of S-ABA 400 mg/L resulted in a higher accumulation of total anthocyanins in the skin of berries and increased the gene expression of CHI, F3H, DFR, and UFGT and of the VvMYBA1 and VvMYBA2 transcription factors in the seedless grape cultivar.

Objetivou-se com este trabalho avaliar a variabilidade dos depósitos de traçadores, simulando herbicidas aplicados em pós-emergência, em populações de Brachiaria plantaginea e Commelina benghalensis infestantes da cultura da soja. Os depósitos dos traçadores foram também avaliados em plantas da cultura, utilizando-se o pulverizador de barra tratorizado, com pontas de jato plano da série 110-SF-03, aplicando o volume de 250 L ha-1 de calda preparada com 0,18% de corante Azul Brilhante e 0,18% de Amarelo Saturn Yellow. Os alvos naturais utilizados foram: plantas de soja com 150 repetições; B. plantaginea no estádio de duas a oito folhas, coletadas na linha da cultura com 141 repetições; e B. plantaginea e C. benghalensis nas entrelinhas, com 150 e 50 repetições, respectivamente. Os alvos artificiais foram constituídos por lâminas distribuídas a 0, 12,2 e 22,5 cm da linha da cultura. Após a aplicação, os alvos foram coletados individualmente e lavados com 30, 20 e 15 mL de água deionizada, para soja, lâminas e plantas daninhas, respectivamente. Estas originaram as amostras analisadas em espectrofotômetro, estimando-se o depósito de calda em µL por planta e µL cm² de área foliar. Foram ajustadas curvas de regressão entre os depósitos unitários e as freqüências acumuladas, utilizando-se o modelo de Gompertz. As relações entre os depósitos máximos e mínimos foram de 7, 4, 10 e 6 para soja, C. benghalensis e B. plantaginea na linha e na entrelinha, respectivamente. As plantas de B. plantaginea da entrelinha receberam, em média, 34% a mais de depósito do que as plantas da linha.

Grape juice contains high amounts of anthocyanins, with great potential for substituting synthetic food dyes. Carrier agents used in spray drying entraps anthocyanins, allowing their preservation. This work appraised whey protein/maltodextrin (WM) and soy protein/maltodextrin (SM) blends as alternative carriers for spray drying of grape juice and encapsulation of anthocyanins. The effects of carrier agent concentration (CAC) and ratio protein/carrier agent (R) on grape juice powder properties were evaluated. The grape juice powders presented good solubility, low water content and high anthocyanin retention. WM blends resulted in higher yields and higher anthocyanin retention (from 77.9 to 94%) than SM blends, whereas SM blends leaded to higher encapsulation efficiency (>97%). Increasing CAC and R resulted in brighter powders, but reconstituted juices presented color parameters similar to those of fresh juice. WM and SM were suitable for encapsulating anthocyanins of grape juice, resulting in powders with potential applications in food industry. Practical Applications The grape cultivar BRS violeta contains high levels of anthocyanins and is an alternative to produce antioxidant‐rich and highly colored grape juice. Spray drying is applied for producing powdered grape juice with high anthocyanin content. In this technique, the addition of whey and soy proteins blended with maltodextrin as carrier agents avoid problems such as stickiness, which is negative to process yield and product quality. Moreover, the use of carrier agents in spray drying promotes the microencapsulation of bioactive compounds, allowing their protection and preservation during processing and storage. The grape juice powder from cv. BRS Violeta can be applied in the food industry as a potential substitute for synthetic food dyes, in addition to being a promising additive for incorporating anthocyanins into functional foods.

a b s t r a c tThe objective of this work was to evaluate the incidence of gray mold of the new seedless table grape 'BRS Vitoria' grown under subtropical conditions, during the cold storage. Grape bunches were obtained from a commercial field trained on overhead trellis and located at Marialva, state of Parana (PR) (South Brazil). The trials were conducted on two consecutive seasons, regular and out of season crops of 2013. Grapes were subjected to the following treatments in a cold chamber: (i) cold storage at 0 • (control); (ii) cold storage at 0 • C with SO 2 pad; (iii) cold storage at 0 • and inoculated with Botrytis cinerea suspension; (iv) cold storage at 0 • C with SO 2 pad and inoculated with B. cinerea suspension. The randomized design was used as statistical model with four treatments and five replicates, with 20 bunches per plot. The incidence of gray mold on grapes was evaluated at 30 days after the beginning of cold storage and at 7 days at room temperature after the end of cold storage. Grape physicochemical variables, such as bunch mass, berry firmness, bunch mass loss, skin color, soluble solids (SS), titratable acidity (TA) and SS/TA were evaluated at the beginning and 30 days after the period of cold storage. The results of this study showed that a good reduction of gray mold was achieved by SO2 during cold storage and shelf life periods. Also, bunch mass, berry firmness, mass loss, color, SS, TA and SS/TA of the grape tested was not negatively affected by SO 2 treatment. A good control of gray mold could be achieved on 'BRS Vitoria' table grapes by sulfur dioxide during storage without affecting fruit quality.

Inúmeros fatores estão envolvidos na tecnologia de aplicação de um herbicida, sendo a deposição correta fundamental para que o produto possa expressar sua eficiência. Com o objetivo de avaliar a deposição de uma solução traçante constituída de glyphosate Roundup Ready (0,96 kg e.a. ha-1) + corante FDC-1 (1.500 ppm), foi conduzido um experimento em área semeada com soja transgênica e infestada com amendoim-bravo (Euphorbia heterophylla), localizada em Londrina-PR. As aplicações foram efetuadas em diferentes estádios de desenvolvimento da cultura, correspondendo a 17, 24, 31, 38 e 45 dias após a emergência da soja. Os alvos, plantas de soja, amendoim-bravo e placas na superfície do solo (linha e entrelinha), foram coletados após pulverização, e a solução traçante foi nestes depositada, posteriormente recuperada através de lavagem com agitação em água destilada. As amostras das soluções recuperadas foram submetidas à análise, utilizando-se procedimentos espectrofotométricos, e os resultados de absorbância convertidos para concentração em µL cm-2 e µL por planta. As freqüências acumuladas dos dados originais de depósito foram adequadamente ajustadas segundo modelo de Gompertz, apresentando elevada precisão (R² > 0,95). Os resultados indicaram que o depósito da calda de pulverização nas plantas de soja e amendoim-bravo reduziu progressivamente com o desenvolvimento da cultura e infestação, sugerindo que a maior garantia de eficiência de controle pode ser conseguida com aplicações precoces.

‘Italia’ grape is one of the most important table grape cultivars grown worldwide. Gray mold, caused by Botrytis cinerea Pers. Fr., is one of the most important causes of postharvest decay of table grapes, and the control of this disease is very difficult because postharvest treatments with synthetic fungicides are not allowed in many countries. The objective of this study was to compare different types of pads releasing different doses of SO2 during cold storage to control gray mold in ‘Italia’ table grapes grown under subtropical conditions. Grape bunches were harvested from a commercial field trained on an overhead trellis located at Cambira, state of Parana (PR), South Brazil. The grapes were packed into carton boxes (capacity, 4.5 kg) and subjected to the following SO2 pad treatments (Uvasys®, Cape Town, South Africa) under cold storage (1.0 ± 1 °C) for 50 days: (i) Control; (ii) SO2 slow release pad; (iii) SO2 dual release pad; (iv) SO2 dual release–fast reduced pad; (v) SO2 slow release pad with grapes inoculated with B. cinerea suspension; (vi) SO2 dual release pad with grapes inoculated with B. cinerea suspension; and (vii) SO2 dual release-fast reduced pad with grapes inoculated with B. cinerea suspension. After cold storage, the grape boxes were maintained for 7 days at room temperature (25 °C). The incidence of gray mold on the grapes, firmness, shattered berries, stem browning, as well as other physicochemical variables, such as bunch mass, bunch mass loss, skin color, soluble solids (SS), titratable acidity (TA) and SS/TA were evaluated. Both SO2 dual release pads were highly efficient in preventing the incidence of gray mold in ‘Italia’ grapes packed in clamshells during the 50-day period of cold storage and at room temperature, even with Botrytis-inoculated berries. The SO2 slow release pad showed lower efficiency, but was higher than the control. The SO2 dual release pad treatments provided the best results with respect to stem browning scores (fresh and green stems) during cold storage, and no differences were observed among the treatments with respect to the other physicochemical evaluations.

The objective of this study was to evaluate techniques, such as flower-cluster and berry-cluster thinning at different times, to prevent bunch compactness of 'BRS Vitoria', a new black seedless grape. The experiment was conducted during two consecutive seasons (2013 and 2014) in a commercial vineyard located in Marialva, PR, Brazil. The grapevines were trained in an overhead trellis system, spaced at 2.5 × 5.0 m. The experimental design used was randomized blocks with four replications and six treatments: control (no thinning); flower-cluster thinning or brushing prior to anthesis; and berry-cluster thinning at different times, when berries were 3-6, 7-10, 11-15, or 16-18 mm in diameter. The bunch compactness incidence was evaluated according to the following classification: very loose, medium loose, and very dense bunches. The physicochemical characteristics of bunches and the yield were also evaluated. Data were submitted to analysis of variance, and means were compared using Tukey's test at 5%. Berrycluster thinning when 'BRS Vitoria' berries are between 7 and 18 mm in diameter is efficient for reducing bunch compactness, as it results in a higher incidence of medium loose and a lower incidence of very dense bunches with optimum yield, while flower-cluster thinning or brushing prior to anthesis should be avoided because it promotes higher incidence of very loose bunches with reduced yield.