Large numbers of acoustic signals from single lipid-shelled Definity® microbubbles have been measured using a calibrated microacoustic system and a two population response observed. Theoretical results based on the Mooney-Rivlin strain softening shell model have been used to identify these populations as primary resonant and off-primary resonant scatter. An experimentally measured size distribution was used to provide the initial resting radius for the simulations, and the responses agree well with the experimental data. In this way, the primary resonant or off-primary resonant behavior of a microbubble can be studied, with potential benefits to both signal processing techniques and microbubble manufacture.
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