Introduction
Materials and MethodsThe industrial development and rapid urbanization has led to development of polluted zones discharging potentially toxic compounds in the environment. Especially, indiscriminate use of pesticides resulted in contamination of aquatic system has now become a global problem and is being extensively researched worldwide [1]. Most of the pesticides are genotoxic [2]. Means these chemicals have the potential of causing DNA damage, increased incidences of neoplasia and adverse effect on vitality and progeny of aquatic animals, which may reduce the productivity of aquaculture. Among different groups of pesticides newly introduced synthetic pyrethroids were once considered as relatively safe [2], but researches had reported the genotoxicity of this group of pesticides [3,4]. Micronucleus (MN) test which is one of the useful methods to detect genotoxicity in aquatic animals [5].India is one of the leading carp producers in the world. The most important species being (rohu), and . Among all rohu is the most preferred species and in last decade contribute about 35% of the total carp production in India [6].cyhalothrin on one of the most important fish species of India i.e.Therefore the present investigation aimed at fi .The experimental protocol was approved by the InstitutionalAnimal Ethics Committee. yhalothrin in , the prolonged toxicity experiment was conducted with reference to the guidelines of OECD 204 except the loading density which was higher than the recommended density 1.0 g/l to reduce the chemical loss.
Labeo rohita
Catla catlaCirrhinus mrigala Labeo rohita.
Labeo rohitaHowever, there were no genotoxicity studies of λ-nding the genotoxicity of λ-cyhalothrin in rohu using micronuclei assayTo study the genotoxicity of λ-c Analytical grade λ-cyhalothrin, ethyl 3-aminobenzoate methanesulfonate, Sigma-Aldrich, USA and cyclophosphamide obtained from Sigma-Aldrich,
Chicken infectious anaemia virus (CIAV) is an economically important pathogen affecting poultry industry worldwide. Commercial poultry flocks (26) suspected to be affected with chicken infectious anaemia (CIA) were processed for Polymerase Chain Reaction (PCR) using VP2 gene primers and characterized by partial gene sequencing. The PCR revealed 26.9% (7/26) poultry flocks positive for CIAV. Eleven PCR products of CIAV DNA were sequenced. The partial nucleotide sequence analysis of VP2 genes revealed that 11 field strains had 99.2 to 100% similarity among themselves and with the Indian strains. The VP2 gene sequences of 11 field strains showed 97.5 to 100% similarity to the field strains sequences reported from all over the world. On the basis of partial nucleotide sequencing analysis of VP2 gene, our findings suggest that the viral strains circulating in Haryana have similarity to other Indian strains.
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