Stevia rebaudiana Bertoni that conventionally propagated by seed or by cuttings or clump division which has a limitation of quality and quantity seed material. In present study, callus culture technique was tried to achieve rapid plant multiplication for quality seed material. Callus induction and multiplication medium was standardized from nodal as well as leaf sagments. It is possible to maintain callus on Murashige and Skoog medium supplemented with 6-benzyl amino purine and naphthalene acetic acid. Maximum callus induction was obtained on Murashige and Skoog medium incorporated with 6-benzyl amino purine (2.0-3.0 mg/l) and naphthalene acetic acid (2.0 mg/l) treatments. However, Murashige and Skoog medium containing 2.0 mg/l 6-benzyl amino purine+2.0 mg/l naphthalene acetic acid was found to be the best for callus induction. Higher regeneration frequency was noticed with Murashige and Skoog medium supplemented with 2.0 mg/l 6-benzyl amino purine+0.2 mg/l naphthalene acetic acid. Regenerated plants were rooted better on ¼ Murashige and Skoog strength supplemented with 0.1 mg/l indole-3-butyric acid. The rooted plantlets were hardened successfully in tera care medium with 63 per cent survival rate. The developed protocol can be utilized for mass production of true to type planting material on large scale independent of season, i.e. external environmental conditions.
This article is distributed under the terms of the Creative Commons Attribution License (CC-BY 4.0), which permits any use, distribution and reproduction in any medium, provided the original author(s) and source are credited.-2-JHEP08(2017)035 The CMS collaboration
Bacillus amyloliquefaciens strain KCP2 was isolated from municipal food waste samples collected in Vallabh Vidyanagar, Gujarat, India. Strain KCP2 is noteworthy due to its ability to produce a thermostable, alkaliphilic a-amylase and a protease. These enzymes have importance in several industrial processes including bread making, brewing, starch processing, pharmacy, and textile industries. Whole genome sequencing of strain KCP2 showed that the estimated genome size was 3.9 Mb, the G ? C content was 46%, and it coded for 4113 genes.
In present study forty-five crosses produced from 9 lines and 5 testers using line × tester mating design were evaluated in Randomized Block Design. Estimation of general combining ability (GCA) gives important information for the selection of parents in terms of performance of hybrids and elucidate the nature and magnitude of gene action involved in the expression of quantitative characters. Present study was caried out to determine the type of gene action and identify inbred lines and crosses with good combining ability for yield and its contributing traits. Variance due to specific combining ability (SCA) was larger than general combining ability (GCA) for the all characters except plant height and ear height. It indicates the preponderance of non-additive gene action in expression of those character except plant height and ear height. Inbred lines WNC 40406 followed by BLD 2, BLD 125 and WNC 40080 showed good combining ability (gca) effect for yield and some of the important yield contributing characters. Among Forty-five hybrids, BLD 2 × BLD 103, WNC 40406 × BLD 125, BLD 114 × IMR 113, WNC 40456 × IMR 113 and BLD 114 × BLD 98 exhibited high specific combining ability (sca) effects for yield. These hybrids are recommended for further evaluation to determine their performance.
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