Abstract. Kuntoro, Sutarti S, Putranto RPA. 2010. Effect of patikan kebo (Euphorbia hirta) extract with bronchial inflammation grade on Balb/C mice with asthma allergic model. . This research aimed to determine the effect of patikan kebo extract towards bronchial inflammation grade on Balb/C mice asthma allergic model. This research used a laboratory experiment with a posttest only control group design by using 24 Balb/C male mice, divided into four groups (control group, asthma allergic group, patikan kebo 10 mg/mice, patikan kebo 20 mg/mice). Samples were sensitized on day-0 and day-14 intraperitoneally, and continued in day-21, 23, 25 and 27 aerosol in 30 minutes. In day-28, the bronchus samples were collected, and the bronchial inflammation grade was observed with Hematoxylin-Eosin staining. The obtained data were analyzed statistically with Kruskal-Wallis continued with MannWhitney using the program of SPSS for Windows Release 12.0. The data were analyzed with a margin of significance p<0.05. The grading the inflammation of control group was grade 2 (83.33%) and grade 3 (16.67%). Asthma allergic group was grade 3 (16.67%) and grade 4 (83.33%). Patikan kebo 10 mg/mice group was grade 2 (33.33%) and grade 3 (66.67%). Patikan kebo 20 mg/mice group was grade 2 (33.33%) and grade 3 (66.67%). There was a significant difference between asthma allergic group with patikan kebo 10 mg/mice and patikan kebo 20 mg/mice group in a grade inflammation (p=0.009). There was no significant difference in a grade inflammation between patikan kebo 10 mg/mice group with patikan kebo 20 mg/mice (p=1,000). Patikan kebo extract 10 mg/mice and 20 mg/mice reduced the bronchial inflammation grade in Balb/C mice asthma allergic model.
<p class="AbstractNormal"><strong>Introduction:</strong> Metabolic syndrome is an accumulation of several disorders, one of which is hyperglycemia. <em>Clitoria ternatea</em> is a plant that contains lots of flavonoids and is known to reduce blood glucose levels in metabolic syndrome. This study aimed to determine the effect of butterfly pea extract (<em>Clitoria ternatea</em>) on blood glucose levels in white rats with metabolic syndrome models.</p><p class="AbstractNormal"><strong>Methods: </strong>This research is an experimental study using animal models with pre and post-test control group design method. The number of samples was 30 male white rats which were divided into 5 groups: group 1–negative control group; group 2–positive control group; group 3, group 4, and group 5 were the metabolic syndrome group which was given <em>Clitoria ternatea</em> extract with doses of 100, 200, and 400 mg/kgBW, respectively for 28 days. Measurement of blood glucose levels for all groups was carried out on the 36<sup>th</sup> day as the pre-test and the 64<sup>th</sup> day as the post-test. Blood glucose levels were obtained through retroorbital venous blood which was analyzed with the Easy Touch blood glucose stick meter. One-way ANOVA was used to analyze data, followed by post-hoc LSD test, and paired T-test.</p><p class="AbstractNormal"><strong>Results: </strong>The average post-test random blood glucose levels decreased by 135.16 mg/dl, 163.18 mg/dl, and 168.35 mg/dl. There was a significant difference (p<0.05) between the pre-test and post-test blood glucose levels along with the average blood glucose level amongst groups.</p><p class="AbstractNormal"><strong>Conclusion: </strong>Butterfly pea (<em>Clitoria ternatea</em>) could decrease blood glucose levels and cause further decrease with higher doses.</p>
Pengaruh pemberian ekstrak herba daun sendok (Plantago major) terhadap kadar glukosa darah mencit Balb/C induksi streptozotocin . This research aimed to determine the effect of daun sendok (Plantago major L.) extract on the blood glucose level of Balb/C mice induced by streptozotocin. This study was a laboratory experimental pre and post-test control group design. The subjects used were 16 male mice. Then, mice were induced with streptozotocin by a dose of 65 mg/kg body weight intraperitoneally freshly dissolved in 0.02 M citrate saline buffer. Mice used were mice with blood glucose levels ≥200 mg/dL. Furthermore, mice were divided into 2 groups by a simple random sampling. Group 1 was given metformin in a dose of 1.3 mg/mice/day and group 2 was given daun sendok extract in a dose of 1000 mg/kg body weight/day. At the 2 nd week, it was measured for the glucose levels from the blood of mice tail by a Blood Glucose Stick Meter (Gluco DrTM). The data obtained were statistically analyzed by independent samples t-test by using SPSS Programme for Microsoft Windows release 17.0. The significance was set at p<0.05. The difference of average blood glucose levels pre vs post experiment, metformin group was -145.87 mg/dL and daun sendok group was -85.00 mg/dL (p=0.024). The experiment results showed that daun sendok extract in a dose of 1000 mg/kg body weight/day could reduce the blood glucose levels in Balb/C mice induced by streptozotocin.
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