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SummaryPurified preparations of broad bean wilt virus contained many isometric particles about 25 m!, in diameter. The particles were of three sorts with sedimenta· tion coefficients (S20,w) of 63, 100, and 126, containing 0, 22, and 33% nucleic acid respectively. The particles with S20,w = 126 were infective. The ribonucleic acid of the virus had a molar base content of G 21· 8 : A 30·0 : C 17·6 : U 30·6.Broad bean wilt virus was closely related serologically to PO virus (a virus' isolated from peas in North America), but did not react with antisera to broad bean stain, red clover mottle, squash mosaic, or true broad bean mosaic viruses.

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