Garcinia indica have been used for centuries with claim for its medicinal value by traditional practitioners to treat numerous diseases. The anti-oxidant activity of G. indica fruit which were assessed by determining the total phenolic content, evaluating the 2, 2-diphenyl-1-picrylhydrazyl, (DPPH) activity, superoxide dismutase (SOD) activity, as well as its ability to inhibit Nitric Oxide (NO) and also their effect on H 2 O 2 induced Reactive Oxygen Species (ROS) generation in human monocytic (THP-1) cells was investigated by tracking intensity of a cell permeable fluorescent dye. In addition, High Performance Liquid Chromatography (HPLC) was used to quantify the bioactive constituent of G. indica fruit extract responsible for its biological activity. The fruit extract of G. indica had the highest amount of hydroxycitric acid (HCA) and phenolic content showed potent antioxidant activity. The IC 50 values for DPPH, SOD and NO scavenging activities were 50.34, 37.08 and 34.15 µg mLG 1 , respectively. Garcinia indica fruit extract (200 μg mLG 1) attenuated $60% of H 2 O 2 mediated ROS generation in THP-1 cells. The above data provides evidence that the fruit extract of G. indica is rich in natural anti-oxidants and thus justify its use in folk medicine especially in the management of free radical-mediated disorders.
Background: Calendula officinalis is one of those plants which have medicinal importance due to its flowers (HECO) has pharmacological activity the important pharmacological parameters have been less investigated properly and can be scientifically proved by reverse pharmacology. It was found of interest to evaluate these properties of extract of flowers of Calendula officinalis.Methods: Study was conducted in Albino Wistar rats. For evaluating wound healing activity Excision wound model and Incision wound mode were used containing 4 groups each. The wound contraction was studied by tracing the raw wound area on graph paper. Scar area and time for complete epithelisation were measured. The percentage of wound contraction was recorded. The differences between experimental groups were compared by ANOVA followed by Students “t” unpaired test.Results: In the excision wound healing model, 5% and 10% flowers showed a highly significant reduction in wound area as compared with control. In incision wound model, rats treated with 5% and 10% ointment of Calendula officinalis flowers extract showed highly significant increase in tensile strength as compared to control.Conclusions: It was found that the Calendula extract enhanced the wound healing in both the models as seen by increased synthesis of connective tissue especially collagen. There was a significant increase in the granuloma tissue. In both the models wound contraction and increased tensile strength was found to be statistically significant.
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