Phytochemical Evaluation and Antioxidant Potential of Garcinia indica Fruits on H2O2 Induced Oxidative Stress in THP-1 Cell Line

Abstract

Garcinia indica have been used for centuries with claim for its medicinal value by traditional practitioners to treat numerous diseases. The anti-oxidant activity of G. indica fruit which were assessed by determining the total phenolic content, evaluating the 2, 2-diphenyl-1-picrylhydrazyl, (DPPH) activity, superoxide dismutase (SOD) activity, as well as its ability to inhibit Nitric Oxide (NO) and also their effect on H 2 O 2 induced Reactive Oxygen Species (ROS) generation in human monocytic (THP-1) cells was investigated by tracking intensity of a cell permeable fluorescent dye. In addition, High Performance Liquid Chromatography (HPLC) was used to quantify the bioactive constituent of G. indica fruit extract responsible for its biological activity. The fruit extract of G. indica had the highest amount of hydroxycitric acid (HCA) and phenolic content showed potent antioxidant activity. The IC 50 values for DPPH, SOD and NO scavenging activities were 50.34, 37.08 and 34.15 µg mLG 1 , respectively. Garcinia indica fruit extract (200 μg mLG 1) attenuated $60% of H 2 O 2 mediated ROS generation in THP-1 cells. The above data provides evidence that the fruit extract of G. indica is rich in natural anti-oxidants and thus justify its use in folk medicine especially in the management of free radical-mediated disorders.

Statistical optimization of photo-induced biofabrication of silver nanoparticles using the cell extract of Oscillatoria limnetica: insight on characterization and antioxidant potentiality

“…The antioxidant activities of the nano-preparations are presented as IC 50 values, which is the concentration of the extract that inhibits the formation of DPPH radicals by 50%, as indicated by the linear regression analysis. 28 ABTS radical-scavenging assay. The ABTS radical-scavenging assay was conducted by following the protocol of Re et al 29 The bluish-green ABTS [2,2 0 -azino-bis(3-ethyl benzothiazoline-6sulfonic acid)] radical was generated through the oxidation reaction resulting from mixing ABTS (7 mM) with potassium persulphate (2.45 mM).…”

Acetaminophen Induced Oxidative and Histopathological Alterations in Hepatic Tissue: Protective Effects of Alstonia Scholaris Leaf Extracts

“…7,33 Leaf extracts of A. scholaris have high phenolic, flavonoid and carotene contents and exhibit potent in-vitro antioxidant functions by scavenging free radicals. 12,13 Pre-and post treatments of ethanolic leaf extracts of A. scholaris following APAP administration restored the TAS, TOS and TTH levels in hepatic tissue, whereas aqueous leaf extract treatment failed to restored these parameters. High hepatoprotective potential of ethanolic extract may be due to presence of high polyphenolic content in ethanolic leaf extract as compared to aqueous leaf extract of A. scholaris.…”

“…[9][10][11] Polyphenolic compounds present in the leaf extract of A. scholaris exhibit potent in-vitro total antioxidant capacity and reducing power and also shown scavenging of free radicals like hydroxyl, peroxy and superoxide radicals. 12,13 These phytochemicals also have important role in stabilizing lipid peroxidation thus reduces cellular damage. Therefore, the present study was aimed to investigate the oxidative and histopathological alterations in liver induced by acetaminophen (APAP) toxicity in wistar rats and its protection by different leaf extracts of Alstonia scholaris Linn.…”

Integrated analysis of proteomics‐delineated and metabolomics‐delineated hepatic metabolic responses to (−)‐hydroxycitric acid in chick embryos

“…[13][14][15] (−)-Hydroxycitric acid [(−)-HCA] is the major active ingredient in the fruit rind of Garcinia cambogia. 16 A myriad of health effects have been attributed to (−)-HCA, such as inhibiting fatty acid synthesis, 12 reducing cholesterol content, 17 anti-inflammatory, 18 antioxidative, 19 antidiabetic 20 properties, and regulating gut microbiota 21 in animals and humans. It was found that (−)-HCA effectively inhibited fatty acid synthesis by inhibiting adenosine triphosphate (ATP)-citrate lyase, which blocked the conversion of citrate to acetyl CoA in the cytosol.…”