Background:
A normal phase- High Performance Liquid Chromatographic (HPLC) method was developed for the enantioseparation of Oxomemazine.
Method:
Separation of enantiomers was attained on Amylose Tris (5-chloro-2-methylphenylcarbamate) using n-hexane: Iso-propyl Alcohol (IPA): Diethylamine (DEA) (60: 40: 0.1) as the mobile phase and the peaks were observed at 227nm using PDA detector. The run time of the analysis was set to 30 min.
Results:
Linearity was found in the range 10-50 µgmL-1. The enantiomers were separated at retention times 16.87 min and 21.37 min.
Conclusion:
The developed method was validated as per the ICH guidelines, thus proving the method to be selective, precise and showing linear response of Oxomemazine peak areas. Additionally, the method of chiral separation being understood by docking simulation study. The method was appropriate for analysis of Oxomemazine in the pure form and its formulation.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.