Chiral Separation of Oxomemazine Enantiomers by HPLC Technique and Enantiomeric Separation Mechanism via Docking Studies

Abstract

Background: A normal phase- High Performance Liquid Chromatographic (HPLC) method was developed for the enantioseparation of Oxomemazine. Method: Separation of enantiomers was attained on Amylose Tris (5-chloro-2-methylphenylcarbamate) using n-hexane: Iso-propyl Alcohol (IPA): Diethylamine (DEA) (60: 40: 0.1) as the mobile phase and the peaks were observed at 227nm using PDA detector. The run time of the analysis was set to 30 min. Results: Linearity was found in the range 10-50 µgmL-1. The enantiomers were separated at retention times 16.87 min and 21.37 min. Conclusion: The developed method was validated as per the ICH guidelines, thus proving the method to be selective, precise and showing linear response of Oxomemazine peak areas. Additionally, the method of chiral separation being understood by docking simulation study. The method was appropriate for analysis of Oxomemazine in the pure form and its formulation.

Study of the Enantioselectivity and Recognition Mechanism of Allyl‐β‐CD Modified Organic Polymer Monolithic Capillary Column

“…Capillary HPLC is a chromatographic technique that shares similarities with conventional HPLC but is characterized by the use of capillary columns with smaller internal diameters. This method is highly efficient and widely used for the separation and analysis of complex mixtures of compounds [11][12][13]. Capillary chromatographic columns play a central role in capillary HPLC.…”

Research method of rapid determination of chiral pesticide fenpropathrin enantiomers in fruit and vegetable puree by supercritical fluid chromatography

“…At present, there are a variety of methods, such as GC [5,6], CEC [7,8], GC-MS [9,10], LC-MS/MS [11][12][13], and HPLC [14][15][16], which are widely used in the field of chiral compound separation. Among them, the CEC chromatographic peak shape is good, but the analysis time is long; GC and GC-MS have high sensitivity and good resolution, but mainly analyze compounds with low boiling point and good thermal stability; HPLC features good resolution, but the organic reagents are consumed in large amounts; LC-MS/MS enjoys high accuracy, but the instruments used are expensive.…”