Ly-6C 1 cells constitute 13 6 3% of freshly isolated (CBA 3 C57BL/6)F 1 mouse spleen leukocytes. Three distinct populations were identified: CD3e 1 NK-1.1 2 conventional T cells (6%), CD3e 2 NK-1.1 2 granulocytes (5%) and CD3e 1 NK-1.1 1 T cells (D2%). The CD3e 1 NK-1.1 1 cells displayed a predominantly large granular leukocyte morphology and were the only Ly-6C 1 cell subset identified by MAb 2B6-F2 to spontaneously lyse the NK-sensitive YAC-1 tumour in vitro. On further phenotypic analysis, these cells co-expressed high levels of TCRVb8.1/8.2 and CD11b, moderate levels of CD90 and low levels of CD4 or CD8. The removal of CD4 1 and CD8 1 cells prior to Ly-6C 1 cell sorting showed that it was the CD4 2 CD8 2 double-negative (
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