LY-6C+ natural T (NT) cells mediate immune surveillance against NK-sensitive and NK-resistant transplantable tumours in certain strains of mice

Martiniello, R; Burton, R C; Yc, Smart

doi:10.1002/(sici)1097-0215(19960516)66:4<532::aid-ijc18>3.0.co;2-9

Cited by 6 publications

(2 citation statements)

References 25 publications

(16 reference statements)

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“…It is interesting that mice deficient in Apo E have increased susceptibility to LPS endotoxemic shock [41]. Ly-6C, which is expressed on multiple cell types including macrophages, endothelial, natural killer (NK), and T cells, has been associated with NK cell cytotoxicity [42][43][44][45]. Cofilin is an actin-binding protein important in actin-filament depolymerization crucial in motility and capping [46].…”

Section: Discussionmentioning

confidence: 99%

Differentiation of stress, metabolism, communication, and defense responses following transplantation

Mueller¹,

Ma²,

Lederer

et al. 2003

Journal of Leukocyte Biology

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The biological complexity of allograft rejection and alloantigen-independent mechanisms is poorly understood. Therefore, we analyzed four components of the biological response following transplantation by global gene analysis. A comparative and kinetic approach was used to identify gene expression profiles. Biological processes were assigned to genes displaying the largest alterations in expression. Metabolism, stress response, and cell organization were the predominant, biological processes associated with ischemia and systemic stress. Innate and adaptive immune responses induced a transcriptional shift toward defense and cell communication. The kinetic analysis showed a shift from innate toward adaptive responses in the post-transplant course.

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Section: Discussionmentioning

confidence: 99%

Differentiation of stress, metabolism, communication, and defense responses following transplantation

Mueller¹,

Ma²,

Lederer

et al. 2003

Journal of Leukocyte Biology

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“…Other NK-sensitive targets, including the Fas 1 Jurkat and Fas 2 B16, were also susceptible to Ly-6C 1 splenocyte killing. We have recently shown that Ly-6C 1 cells are also responsible for the immunosurveillance against B16 transplanted into mice (Martiniello et al, 1996). Furthermore, our data suggest that multiple killing mechanisms are utilized by NT cells.…”

Section: Was Responsible For Yac-1 Lysismentioning

confidence: 60%

Natural cell-mediated cytotoxicity (NCMC) against NK-sensitive tumoursin vitro by murine spleen LY-6C+ natural T cells

Martiniello¹,

Burton²,

Smart³

1997

Int. J. Cancer

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Ly-6C 1 cells constitute 13 6 3% of freshly isolated (CBA 3 C57BL/6)F 1 mouse spleen leukocytes. Three distinct populations were identified: CD3e 1 NK-1.1 2 conventional T cells (6%), CD3e 2 NK-1.1 2 granulocytes (5%) and CD3e 1 NK-1.1 1 T cells (D2%). The CD3e 1 NK-1.1 1 cells displayed a predominantly large granular leukocyte morphology and were the only Ly-6C 1 cell subset identified by MAb 2B6-F2 to spontaneously lyse the NK-sensitive YAC-1 tumour in vitro. On further phenotypic analysis, these cells co-expressed high levels of TCRVb8.1/8.2 and CD11b, moderate levels of CD90 and low levels of CD4 or CD8. The removal of CD4 1 and CD8 1 cells prior to Ly-6C 1 cell sorting showed that it was the CD4 2 CD8 2 double-negative (

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Purine nucleoside phosphorylase and fludarabine phosphate gene‐directed enzyme prodrug therapy suppresses primary tumour growth and pseudo‐metastases in a mouse model of prostate cancer

Martiniello‐Wilks

Wang

Voeks

et al. 2004

The Journal of Gene Medicine

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Gene-directed enzyme prodrug therapy based on the E. coli purine nucleoside phosphorylase (PNP) gene produces efficient tumour cell killing. PNP converts adenosine analogs into toxic metabolites that diffuse across cell membranes to kill neighbouring untransduced cells (PNP-GDEPT). Interference with DNA, RNA and protein synthesis kills dividing and non-dividing cells, an important consideration for slow-growing prostate tumours. This study examined the impact of administering PNP-GDEPT into orthotopically grown RM1 prostate cancers (PCas) on the growth of lung pseudo-metastases of immunocompetent mice. C57BL/6 mice bearing orthotopic RM1 PCas received a single intraprostatic injection of OAdV220 (10(10) particles), a recombinant ovine atadenovirus containing the PNP gene controlled by the Rous Sarcoma virus promoter, followed by fludarabine phosphate (approximately 600 mg/m(2)/day) administered intraperitoneally (ip) once daily for 5 days. Pseudo-metastases were induced 2 days after intraprostatic vector administration by tail-vein injection of untransduced RM1 cells. Mice given PNP-GDEPT showed a significant reduction both in prostate volume (approximately 50%) and in lung colony counts (approximately 60%). Apoptosis was increased two-fold in GDEPT-treated prostates compared with controls (P < 0.01), but was absent in the lungs. Staining for proliferating cell nuclear antigen (PCNA) indicated that proliferation of both RM1 prostate tumours (P < 0.01) and lung colonies (P < 0.01) was significantly suppressed after GDEPT. Although prostate tumour immune cell infiltration did not differ significantly between treatments, immunostaining for Thy-1.2 (CD90) showed that GDEPT promoted Thy-1.2(+) cell infiltration into the prostate tumour site. This study showed that a single course of PNP-GDEPT significantly suppressed local PCa growth and reduced lung colony formation in the aggressive RM1 tumour model.

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LY-6C+ natural T (NT) cells mediate immune surveillance against NK-sensitive and NK-resistant transplantable tumours in certain strains of mice

Abstract: We have previously shown that anti-Ly-6C monoclonal antibody (MAb) 2B6-F2 identifies a subset of (CBA % o 1996 Wiley-Liss, Inc.C57BL

Cited by 6 publications

References 25 publications

Differentiation of stress, metabolism, communication, and defense responses following transplantation

Differentiation of stress, metabolism, communication, and defense responses following transplantation

Natural cell-mediated cytotoxicity (NCMC) against NK-sensitive tumoursin vitro by murine spleen LY-6C+ natural T cells

Purine nucleoside phosphorylase and fludarabine phosphate gene‐directed enzyme prodrug therapy suppresses primary tumour growth and pseudo‐metastases in a mouse model of prostate cancer

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