The aim of this study was to evaluate the relationship between plasma leptin and lipid levels in breeding mares and their newborn foals. The study was conducted on 17 Polish cold-blood mares and their newborn foals. The mares were divided into two groups, according to the course of delivery. These groups were seven mares which had a normal delivery and 10 mares which had required veterinary intervention during their delivery. Blood samples were taken from the jugular vein of both the mares and their foals. The blood samples were taken within 30 min after delivery, as well as on four successive mornings directly afterwards. In obtained plasma samples, the leptin, triacylglycerols (TG) and free fatty acid (FFA) concentrations were measured. The leptin and TG levels in the plasma sampled on the day of delivery and the two following days were significantly higher in mares which received veterinary intervention during the delivery, when compared to the results obtained from the plasma of mares which had normal delivery. The results obtained from foals did not show any statistical differences. In conclusion, the conducted tests have shown that post-parturient hyperlipidemia and hyperleptinemia in mares did not influence the lipid status and plasma leptin level in newborn foals.
the aim of the present study was to determine virulence factors and antibiotic susceptibility of Staphylococcus xylosus isolated from subclinical mastitis in cows. the material consisted of 42 isolates of S. xylosus obtained from 276 samples of milk collected from cows with subclinical mastitis. the isolates were obtained from the udder secretions of 33 cows from farms in the lublin region (Poland). S. xylosus was found in 15.2% of tested milk samples. the study did not reveal any macroscopic changes in the milk or symptoms in the cow's body. the number of somatic cells in milk samples ranged from 245,000 to 416,000/ml of milk (on average 268,000/ml of milk). the ability to produce slime was observed in 42.9% of S. xylosus isolates. none of the tested isolates demonstrated the ability to produce protease or cause haemolysis. five isolates of S. xylosus (11.9%) were classified to the methicillin-resistant group. The meca gene was not found in any of these isolates. The enzyme β-lactamase was detected in 28.6% of S. xylosus isolates. The highest efficacy against S. xylosus was demonstrated for cephalosporin antibiotics: cefacetrile and cefoperazone (80.1% and 76.2% of susceptible isolates of S. xylosus, respectively). A significant quantity of isolates was resistant to streptomycin, linkomycin, penicillin and neomycin (approximately 10% of susceptible isolates of S. xylosus).
Searching for new more accurate biomarkers in order to evaluate the health of bitches with reproductive disorders and disturbances has been a subject of inquiry for many researchers. Biomarkers are measurable indicators, the concentrations of which are dependent on the degree and type of stimulus. Serum C-reactive protein (CRP) has been proven to be a valuable biomarker for health status evaluation of bitches suffering from reproductive disorders, such as pyometra, as well as for treatment monitoring. However, no data are available related to the CRP profile in bitches with ovarian cysts for a better evaluation of their condition and prognosis. Thus, the aim of this study was to determine the possible usefulnessof CRP determination in the serum of bitches with ovarian cysts. Our results suggest that CRP concentrations could be useful indicators of the presence of ovarian cysts, although studies with more animals are needed..
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