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SUMMARYThe aim of this study was to evaluate a manual method for concentrating canine platelets and consequently produce autologous platelet concentrates (APCs) for clinical and experimental proposals. The APCs were obtained by venous blood collection in tubes with ACD solution and were spun at 191 g for 6 minutes. The cell counts of whole blood and APCs, were statistically different (P < 0.01) between the values for platelets, leukocytes, granulocytes, monocytes, but not for lymphocytes. A strong negative correlation (ρ = -0.702 P = 0.011) between the number of lymphocytes and platelets in the APCs was found. The collection efficiency of platelets was 29.9% and the concentration of platelets was 49.4% higher in APCs when compared to the whole blood samples. The results indicated that this simple centrifugation method allows concentrating canine platelets.Palabras clave: perro, plasma rico en plaquetas autólogo, medicina regenerativa, método del tubo.

SUMMARYCranial cruciate ligament rupture (CCLR) is one of the main orthopedic problems that could produce hind limb lameness in dogs. Even though this problem can be surgically treated the development and progression of osteoarthritis are typical features. A case of a dog that received postoperative intra-articular injections of autologous platelet concentrates (APCs) after the surgical reparation of a CCLR is described. The clinical, radiographic, and gait analysis results could potentially support the postoperative use of intra-articular injections of APCs as an adjunctive therapy in the CCLR surgical reparation.Palabras clave: perro, rotura del ligamento cruzado anterior, plasma rico en plaquetas, terapia regenerativa.