No abstract

The objective of this study was to determine the developmental rates and relative abundance of Hsp 70.1 and Glut-1 transcripts in in vivo- and in vitro-produced (IVP) bovine embryos in media supplemented with bovine serum albumin (BSA) or different oestrous cow serum concentrations. In experiment 1, in vitro maturation and culture media were supplemented with 0.4% BSA or 1, 5, 10 or 20% of oestrous cow serum (ECS). The analysis of the expression of Hsp 70.1 and Glut-1 was carried out in individual days 7 and 8 embryos by a semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) assay. In experiment 2, in vivo-produced morulae were collected on day 7 of the oestrous cycle and employed for the comparison of the relative abundances of Hsp 70.1 and Glut-1 transcripts with IVP morulae produced using two protein sources (10% ECS or 0.4% BSA). No differences were observed in cleavage rate among groups, but blastocyst formation (27%) and hatching rates (78%) were significantly higher in IVP embryos produced with 20% ECS than the other groups (p<0.05). No significant differences were observed in the relative abundances of Hsp 70.1 and Glut-1 mRNA in days 7 and 8 blastocysts expanded blastocysts between groups. The abundances of mRNA for those genes were similar between IVP and in vivo-produced morulae. In spite of the alterations observed in embryonic development, the presence of serum at distinct concentrations did not appear to alter the relative abundance profiles of Hsp 70.1 and Glut-1 compared with controls or the BSA supplementation to the IVP media.

We report on magneto-optical imaging, magnetization, Hall effect and magnetoresistance experiments in Nb/Al 2 O 3 /Co thin film heterostructures.The magnetic field is applied perpendicularly to the plane of the film and gives rise to abrupt flux penetration of dendritic form. A magnetization texture is imprinted in the Co layer in perfect coincidence with these ramifications. The spin domains that mimic the vortex dendrites are stable upon the field removal. Moreover, the imprinted spin structure remains visible up to room temperature. Complementary magnetization, Hall effect and magneto-resistance experiments were performed in a similar sample where electrical contacts were placed on the Co layer. In the region of the field -temperature diagram where flux instabilities are known to occur in Nb films, irregular jumps are observed in the magnetic hysteresis and large amplitude noise is detected in the magneto-resistance and Hall resistivity data when measured as a function of the field.

An in-field interaction plot, δM R , has been recently introduced, presenting important advantages over the classical remanence plots. Here a general δM R is proposed, allowing to assess interactions even in systems with shifted and asymmetric major loops. To construct such a plot, a recoil loop and the position of the center of the major loop are only needed. Applying the method on exchange-biased Co/IrMn bilayer gives two types of δM R obtained for measuring field either parallel or antiparallel to the exchange-bias direction. This provides valuable information on the reversal mechanism and allows distinguishing between effects coming from magnetic coupling into the ferromagnet (Co) and those stemming from interactions in its interface with the antiferromagnet (IrMn). The essentially nonzero general δM R plot obtained from the major loop revealed to be a precise measure of the reversal asymmetry. The technique can readily be adjusted for use in other scientific fields where hysteresis is observed. We provide free software which generates such δM R plot(s).PACS numbers: 75.70.Cn, 75.30.Gw, 75.30.Et Wohlfarth pointed out a simple relation 1 between remanence curves of systems with symmetric major magnetization M , versus magnetic field H, hysteresis loops. These are the isothermal remanent magnetization curve M r (H), which represents the remanence obtained by the application and removal of a positive field on an initially demagnetized sample, and the DC demagnetization curve M d (H), i.e., the remanence resultant from the application of a negative field to a sample initially at saturation remanence. The Wohlfarth's relation