Splitting and biopsy for bovine embryo sexing under field conditions

Lopes, R.F.; Forell, Fabiana; Oliveira, Alexandre Tavares Duarte de; Rodrigues, José Ariévilo Gurgel

doi:10.1016/s0093-691x(01)00641-0

Cited by 58 publications

(53 citation statements)

References 31 publications

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“…In this study, the gender was determined in 91.3% of embryos for fresh transfer and in 87.5% of embryos intended for freezing (Table 1), which accords with our previous work with whole, intact (88.7%; Lopatarova et al, 2007) and splitted embryos (89.4-92%; Lopatarova et al, 2008). Our results are similar to those published by other authors (85-95%) using embryos from superovulated donors (Thibier and Nibart, 1995;Shea, 1999;Li et al, 2007;Yu et al, 2007) as well as IVP embryos (Lopes et al, 2001;Hasler et al, 2002;Tominaga and Hamada, 2004). However, the successful sex detection was inversely related to the number of isolated embryonic cells (≤ 3 cells 85.5%, 4-6 cells 97.4%, ≥ 7 cells 100%; Lacaze et al, 2008).…”

Section: Discussionsupporting

confidence: 91%

“…Comparison of ethylene glycol or glycerol as cryoprotectants did not show any influence on the pregnancy rate (Shea, 1999;Lopes et al, 2001). We used only 1.5M ethylene glycol for a rapid and effective thawing procedure.…”

Section: Discussionmentioning

confidence: 98%

“…The viability of fresh biopsied embryos after ET varies from 49 to 62% (Lopes et al, 2001;Hasler et al, 2002;Li et al, 2007) using the various mode of biopsy. Our result in pregnancy rate (Table 2) was comparable with published data and does not differ between the pregnancy rates after ET of fresh intact (control 1, 61.9%) and sexed embryos (56.5%).…”

Section: Discussionmentioning

confidence: 99%

“…Better results were published later using PCR amplification of specific DNA sequences to determine embryonic sex in cattle (Herr et al, 1990;Schroder et al, 1990;Peura et al, 1991), pigs (Pomp et al, 1995), horses (Peippo et al, 1995), humans (Handyside et al, 1990) and mice (Han et al, 1993). Recently published results support the suitability of the PCR method for sex determination in cattle on the basis of its high accuracy and quick results (Thibier and Nibart, 1995;Lopes et al, 2001;Ekici et al, 2006;Yu et al, 2007).…”

mentioning

confidence: 99%

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Conception rate after sex determination and cryopreservation of D7 bovine embryos:

Lopatářová¹,

Čech²,

Krontorád³

et al. 2010

Vet. Med. - Czech

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ABSTRACT:The aim of this study was to evaluate conception rate after sex determination and subsequent freezing of Day 7 (D 7 ) bovine embryos and to compare it with transfer of fresh female embryos. High quality embryos obtained from superovulated donors were biopsied and the embryonic cells (5-10) were analysed by PCR using specific primers for the Y-chromosome determinant. Fresh embryos were blade biopsied (n = 172) and subsequently transferred (ET) ipsilaterally to synchronized recipients. Selected embryos (n = 112) were biopsied by cell aspiration and cryopreserved for later transfer. Sex determination was successfully completed in 91.3% of fresh group embryos (44% female) and in 87.5% of freeze-thawed group embryos (45.9% female). The achieved pregnancy rate was 56.5% after fresh transfer of sexed (female) embryos whereas intact embryos (control 1) implanted in 61.9% recipients. 95.6% (43/45) of embryos survived biopsy and cryopreservation. After transfer of freeze-thawed sexed (female) embryos, pregnancy was established in 48.8% of recipients. A similar conception rate was found in intact embryos (50.7%, control 2). The results clearly demonstrate that the microsurgical technique used and subsequent PCR sex analysis allow the rapid commercial exchange of genetic resources on the basis of fresh or frozen sex-desired embryos in embryo transfer programs.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Conception rate after sex determination and cryopreservation of D7 bovine embryos:

Lopatářová¹,

Čech²,

Krontorád³

et al. 2010

Vet. Med. - Czech

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“…There are various reports of livestock pregnancy rates of 30% to 60% when using biopsied embryos, similar to the rates obtained with intact embryos and considered sufficient for commercial applications (Agca et al, 1998, Chrenek et al 2001, Garcia, 2001, Lopes et al 2001, Park et al 2001, Chen et al 2002, Tominaga, 2004, Tominaga and Hamada, 2004.…”

Section: Introductionmentioning

confidence: 64%

A deterministic simulation study of embryo marker-assisted selection for age at first calving in Nellore (Bos indicus) beef cattle

et al. 2007

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We used deterministic simulation of four alternative multiple ovulation and embryo manipulation (MOET) closed nucleus schemes to investigate the benefits of using marker-assisted selection (MAS) of Nellore (Bos indicus) beef cattle embryos prior to transplantation to reduce the age at first calving (AFC). We found that MAS resulted in increased genetic gain as compared to selection without AFC quantitative trait loci (AFC-QTL) information. With single-stage selection the genetic response (GR) increased as follows: GR = 0.68% when the AFC-QTL explained 0.02 of the AFC additive genetic variance (σ A 2 ); GR = 1.76% for AFC-QTL explaining 0.05 σ A 2 ; GR = 3.7% for AFC-QTL explaining 0.1 σ A 2 ; and GR = 55.76% for AFC-QTL explaining 0.95 σ A 2 . At the same total selected proportion, two-stage selection resulted in less genetic gain than single stage MAS at two-years of age. A single stage selection responses of ≥ 95% occurred with pre-selected proportions of 0.4 (0.1 σ A 2 explained by AFC-QTL), 0.2 (0.3 σ A 2 explained by AFC-QTL) and 0.1 (0.5 σ A 2 explained by AFC-QTL), indicating that the combined use of MAS and pre-selection can substantially reduce the cost of keeping recipient heifers in MOET breeding schemes. When the number of recipients was kept constant, the benefit of increasing embryo production was greater for the QTL explaining a higher proportion of the additive genetic variance. However this advantage had a diminishing return especially for QTL explaining a small proportion of the additive genetic variance. Thus, marker assisted selection of embryos can be used to achieve increased genetic gain or a similar genetic response at reduced expense by decreasing the number of recipient cows and number of offspring raised to two-years of age.

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