Seminal oxidative stress (OS) is well-known to affect male fertility status. The discrepancy in OS measurement has hindered its clinical use as a quality indicator for semen. Some tests measured single markers of oxidants or reductants, leading to lack of standardization of results. Oxidation-reduction potential (ORP) is a better representative for OS as it provides an overall measure of the activity of both oxidants and reductants. ORP assessment by MiOXSYS has been introduced as a measure of OS with high specificity in differentiating fertile from infertile semen samples. This is a retrospective study comparing data from semen analysis and ORP measurements between two andrology laboratories in the USA and Qatar over a period of 12 months. The same protocol was followed by both laboratories. The USA dataset contained 194 patients and 51 fertile donors, while the Qatar dataset contained 400 patients and 50 fertile donors. In both datasets and in the combined dataset, the infertile group had significantly lower sperm concentration, total and progressive motility, and normal morphology as well as higher ORP levels compared to fertile men (p< 0.05). When comparing data from both centers, the infertile group showed significant difference between both datasets regarding progressive motility and morphology (p < 0.001). Also, the percentage of patients with abnormal semen volume, sperm count, total and progressive motility were significantly different between both datasets (p < 0.05). ORP levels showed no significant difference between both datasets (p < 0.08). ROC analysis indicated that ORP cutoff value of 1.42 mV/10 /mL in the USA group, Qatar group, and combined dataset can accurately differentiate fertile from infertile semen groups. Although other semen parameters showed significant differences between the two centers, ORP remained consistent in both datasets individually or in combined data. This proves its reproducibility and reliability.
OBJECTIVE: Oxidation reduction potential (ORP) is a new tool to measure oxidative stress (OS) and has been shown to serve as an accurate predictor of poor semen quality in infertile men. Recent studies have shown that ORP test is a simple, quick, inexpensive and reproducible test of OS status in semen. Assessment of sperm DNA fragmentation (SDF) has been shown recently to correlate with fertility outcome in spontaneous pregnancy and assisted reproduction. OS is known as a major cause of SDF. We therefore, set out to investigate the relationship between the ORP and SDF in patients with male infertility. DESIGN: Retrospective cohort study. MATERIALS AND METHODS: Study included 309 infertile patients (Group 1) and 47 normal fertile donors (controls; Group 2) between Jan to Jun 2016 at a tertiary medical center. Patients with azoospermia, leukocytospermia, history of smoking, sexually transmitted diseases or those receiving antioxidants were excluded. Data on medical history, physical examination, semen analysis, ORP and SDF testing was collected.RESULTS: The mean age of all subjects was 35.6 AE 7.85; and there were no differences between the two groups. Patients had significantly higher ORP (2.74 AE 3.92 vs. 1.26 AE 1.12 mV/10 6 sperm/mL; P<0.001) and SDF (27.6 AE 17.8% vs. 15.68 AE 6.31%, P<0.001) than controls. ORP levels correlated significantly with SDF in all subjects (r ¼ 0.256; P<0.001) and in patients group (r ¼ 0.222; P<0.001).CONCLUSIONS: Correlation of ORP levels with SDF confirms the causal relationship between OS and SDF. ORP could be used as a surrogate marker for SDF in clinics which lack access to highly complex sperm function testing due to the expense or need for highly trained laboratory personnel.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.