In this study, we examined the effects of superstimulation using follicle‐stimulating hormone (
FSH
) followed by gonadotropin‐releasing hormone (Gn
RH
) on buffalo embryo production by ultrasound‐guided ovum pick‐up (
OPU
) and in vitro fertilization (
IVF
). Nine Murrah buffaloes were subjected to
OPU
‐
IVF
without superstimulation (control). The morphologies of the oocytes collected were evaluated, and oocytes were then submitted to in vitro maturation (
IVM
). Two days after
OPU
, same nine buffaloes were treated with twice‐daily injections of
FSH
for 3 days for superstimulation followed by a Gn
RH
injection. Oocytes were collected by
OPU
23–24 hr after the Gn
RH
injection and submitted to
IVM
(the superstimulated group). The total number of follicles, number of follicles with a diameter > 8 mm, and number of oocytes surrounded by multi‐layered cumulus cells were higher in the superstimulated group than in the control group (
p
≤ 0.05). After
IVF
, the percentages of cleavage and development to blastocysts were higher in the superstimulated group than in the control group (
p
< 0.05). In conclusion, superstimulation improved the quality of oocytes and the embryo productivity of
OPU
‐
IVF
in river buffaloes.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.