In this study, we examined the effects of superstimulation using follicle‐stimulating hormone ( FSH ) followed by gonadotropin‐releasing hormone (Gn RH ) on buffalo embryo production by ultrasound‐guided ovum pick‐up ( OPU ) and in vitro fertilization ( IVF ). Nine Murrah buffaloes were subjected to OPU ‐ IVF without superstimulation (control). The morphologies of the oocytes collected were evaluated, and oocytes were then submitted to in vitro maturation ( IVM ). Two days after OPU , same nine buffaloes were treated with twice‐daily injections of FSH for 3 days for superstimulation followed by a Gn RH injection. Oocytes were collected by OPU 23–24 hr after the Gn RH injection and submitted to IVM (the superstimulated group). The total number of follicles, number of follicles with a diameter > 8 mm, and number of oocytes surrounded by multi‐layered cumulus cells were higher in the superstimulated group than in the control group ( p ≤ 0.05). After IVF , the percentages of cleavage and development to blastocysts were higher in the superstimulated group than in the control group ( p < 0.05). In conclusion, superstimulation improved the quality of oocytes and the embryo productivity of OPU ‐ IVF in river buffaloes.
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