An experiment was conducted to determine the effect of high dietary intakes of Zn and Cu and their combination on growth performance of weanling pigs with diverse health status and management strategies. Twelve experiment stations cooperated and used a total of 1,356 pigs that averaged 6.55 kg BW and 22.2 d age at weaning. The four dietary treatments, all of which met or exceeded NRC requirements, were 1) control, 2) 3,000 ppm Zn (from Zn oxide), 3) 250 Cu ppm (from Cu sulfate), or 4) 3,000 ppm Zn and 250 ppm Cu. The diets were fed as a complex Phase I diet (1.4% lysine) for 7 d followed by a Phase II diet (1.2% lysine) for 21 d. Chlortetracycline (220 ppm) was added to all diets. Fecal color (1 = yellow to 5 = black) and consistency (1 = very firm to 5 = very watery) were scored daily for 3 wk. At the end of the 28-d study, 412 pigs were bled at five stations, and plasma Cu, Zn, and Fe concentrations were determined at one station with atomic absorption spectrophotometry. Average daily gain (375, 422, 409, 415 g/d), feed intake (637, 690, 671, 681 g/d), and gain/feed (586, 611, 611, 612 g/kg) were improved (P < .01) by the addition of Zn and(or) Cu. Significant Cu x Zn interactions imply that the responses to Zn and Cu were independent and not additive. There were significant (P < .01) Zn and Cu effects and a Zn x Cu interaction on fecal color (3.17, 3.24, 4.32, 3.57) and consistency (2.39, 2.14, 2.14, 2.13). Dietary additions of Cu and Zn resulted in elevated plasma concentrations of Cu and Zn, respectively. These data indicate that pharmacological additions of 3,000 ppm Zn (oxide) or 250 ppm Cu (sulfate) stimulate growth beyond that derived from intakes of Zn and Cu that meet nutrient requirements. However, the combination of Zn and Cu did not result in an additive growth response.

Conjugated linoleic acid (CLA) is a collective term for positional and geometric isomers of linoleic acid. Dietary CLA has been shown to improve feed efficiency, decrease body fat, and increase lean tissue in laboratory animals. We hypothesized that CLA would improve performance and carcass composition and would be deposited in pork tissues. Diets of 40 crossbred pigs were supplemented with CLA to determine its effects on performance and carcass composition. Eight replications of five littermate barrows with an initial average weight of 26.3 kg were allotted at random to individual pens. Within replication dietary treatments containing 0, 0.12, 0.25, 0.5, or 1.0% CLA were assigned at random. Pigs were weighed and feed disappearance was determined at 14-d intervals. Average daily gain increased linearly as the level of CLA increased in the diet (P < 0.05). Average daily feed intake was not affected by the concentration of CLA in the diet. Therefore, a linear increase in gain:feed ratio (P < 0.05) was observed. Carcasses from animals fed control diets had greater 10th rib backfat than carcasses from animals fed CLA (P < 0.05). Ultrasound measurement and carcass measurements showed less fat depth over the loin eye at the 10th rib of pigs fed doses of CLA (P < 0.05) than that observed for control pigs. Belly hardness (firmness) increased linearly as the concentration of CLA in the diet increased when bellies were measured for firmness either lean side up (P < 0.001) or lean side down (P < 0.05). Loin dissection data demonstrated that CLA produced a quadratic treatment effect both for less intermuscular fat (P < 0.001) and less subcutaneous fat (P < 0.05) and a linear increase for bone (P < 0.05), although finished loin weight only tended to increase (P = 0.08). The CLA concentration increased in a linear relationship in both subcutaneous fat (P < 0.001) and lean tissue (P < 0.001). Dietary CLA was incorporated into pig tissues and had positive effects on performance and body composition.

A study involving nine research stations from the NCR-42 Swine Nutrition Committee used a total of 1,978 crossbred pigs to evaluate the effects of dietary ZnO concentrations with or without an antibacterial agent on postweaning pig performance. In Exp. 1, seven stations (IA, MI, MN, MO, NE, ND, and OH) evaluated the efficacy of ZnO when fed to nursery pigs at 0, 500, 1,000, 2,000, or 3,000 mg Zn/kg for a 28-d postweaning period. A randomized complete block experiment was conducted in 24 replicates using a total of 1,060 pigs. Pigs were bled at the 28-d period and plasma was analyzed for Zn and Cu. Because two stations weaned pigs at < 15 d (six replicates) and five stations at > 20 d (18 replicates) of age, the two sets of data were analyzed separately. The early-weaned pig group had greater (P < 0.05) gains, feed intakes, and gain:feed ratios for the 28-d postweaning period as dietary ZnO concentration increased. Later-weaned pigs also had increased (P < 0.01) gains and feed intakes as the dietary ZnO concentration increased. Responses for both weanling pig groups seemed to reach a plateau at 2,000 mg Zn/kg. Plasma Zn concentrations quadratically increased (P < 0.01) and plasma Cu concentrations quadratically decreased (P < 0.01) when ZnO concentrations were > 1,000 mg Zn/kg. Experiment 2 was conducted at seven stations (KY, MI, MO, NE, ND, OH, and OK) and evaluated the efficacy of an antibacterial agent (carbadox) in combination with added ZnO. The experiment was a 2 x 3 factorial arrangement in a randomized complete block design conducted in a total of 20 replicates. Carbadox was added at 0 or 55 mg/kg diet, and ZnO was added at 0, 1,500, or 3,000 mg Zn/ kg. A total of 918 pigs were weaned at an average 19.7 d of age. For the 28-d postweaning period, gains (P < 0.01), feed intakes (P < 0.05), and gain:feed ratios (P < 0.05) increased when dietary ZnO concentrations increased and when carbadox was added. These responses occurred in an additive manner. The results of these studies suggest that supplemental ZnO at 1,500 to 2,000 mg Zn/kg Zn improved postweaning pig performance, and its combination with an antibacterial agent resulted in additional performance improvements.

A cooperative research study involving three experiments and 2,318 pigs was conducted at 12 research stations to evaluate the protein (lysine) requirements of barrows and gilts. The two sexes were penned separately and fed fortified corn-soybean meal diets containing protein levels ranging from 12.0 to 17.2%. Lysine levels in these diets ranged from .52 to .90%. Protein levels in Exp. 1 were 12, 14, and 16%; in Exp. 2, protein levels were 13, 14, 15, and 16%; and in Exp. 3, they were 13.2 15.2, and 17.2%. Fat (5%) was added to one-half of the diets in Exp. 3. Each station that participated contributed a minimum of two replicate pens of pigs per diet-sex combination in a given experiment. Average initial and final weights were 35 and 99 kg in Exp. 1 and 51 and 105 kg in Exp. 2 and 3, respectively. At the end of the test period, pigs were slaughtered and hot carcass weight, 10th rib fat depth, and longissimus muscle area were measured. Percentage of carcass muscle was estimated from these data. Overall, barrows gained weight faster than gilts (P < .01), but gilts required less feed per unit of gain (P < .05) and had less backfat, larger longissimus muscle areas, and a greater percentage of carcass muscle (P < .01) than did barrows. Lean growth rate was similar for barrows and gilts (332 vs 329 g/d). Increasing the dietary protein or lysine level resulted in improved rate and efficiency of gain and increased carcass leanness and lean growth rate in gilts, but the increase was less pronounced or did not occur in barrows, resulting in protein level x sex interactions. Feeding low-protein (12 or 13%) diets decreased performance and carcass leanness to a greater extent in gilts than in barrows. The pooled data from the three experiments indicated that most traits tended to reach a plateau at 13% CP (.60% lysine) in barrows, whereas in gilts, weight gains, feed/gain, carcass muscle, and lean growth rate continued to improve, but at a decreasing rate, with up to 17.2% CP (.90% lysine). The results indicate that gilts require higher concentrations of dietary amino acids to maximize lean growth rate than do barrows.

A cooperative research study involving members of a regional committee (North Central Regional Committee on Swine Nutrition [NCR-42]) was conducted to assess the variability in nutrient composition (DM, CP, Ca, P, Se, and amino acids) of corn and soybean meal from 16 sources (15 states, mostly in the Midwest) and to assess the analytical variability in nutrient assays among 22 laboratories (labs; 16 experiment station labs and six commercial labs). Corn samples were obtained from each participating station's feed mill during a 3-yr period (1989, 1990, and 1992), as were soybean meal samples during a 2-yr period (1989 and 1990). Both regular soybean meal (with hulls) and dehulled soybean meal were represented in the study. Samples were analyzed for DM and CP by all 22 labs, for Ca and P by 15 labs, for amino acids by 10 labs, and for Se by 6 labs. Each lab used its own analytical procedures. Samples of corn and soybean meal varied in their nutrient composition depending on the area of origin. The greatest variation among sources was in Se concentration, ranging from .02 to .29 mg/kg in corn and .08 to .95 mg/kg in soybean meal. Crude protein and lysine in corn were positively correlated, but the regression coefficient was low (r2 = .49). The relationship between CP and lysine for the two soybean meals combined was considerably stronger (r2 = .81). Lysine in corn increased by .018 percentage point and lysine in soybean meal (regular and dehulled combined) increased by .063 percentage point for each 1 percentage point increase in CP. Except for CP and Se, the analytical variability among labs was as great as, and in some cases greater than, the variability in nutrient composition among sources of corn and soybean meal. Within-lab analytical variability tended to be less than among-lab variability. Some labs performed certain analyses with considerably less variability and more accuracy than others.