A method has been developed for the determination of hydroxyproline by the Technicon AutoAnalyserl using a modified version of the Stegemann assay (1958). This involves oxidation of the hydroxyproline with chloramine T; excess chloramine T is destroyed with perchloric acid and the hydroxyproline chromogen is then reacted with p-dimethylaminobenzaldehyde to develop a pink colour. In the present method the absorption is measured at 550 my. The reagents used are adapted from the method described by Woessner (1961), in particular the composition of the p-dimethylaminobenzaldehyde solution (P.D.A. reagent) has been changed to avoid precipitation in the transmission tubing. This method has been applied to the determination of collagen and elastin in arteries and connective tissues and the results obtained agree well with those found using a manual method.
Ten patients with chronic epilepsy were selected; five had high and five had low total serum testosterone levels. Each patient slept for two nights in the sleep laboratory. EEG monitoring and measurements of nocturnal penile tumescence were carried out during sleep. A clear relationship between both total and free serum testosterone levels were found with nocturnal penile tumescence. FSH, LH, SHBG, and prolactin showed no relationship. The five patients with low serum testosterone levels all showed reduced sexual activity.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.