Aim: This study was carried out to investigate the Susceptibility of Candida albicans, Staphylococcus aureus and Escherichia coli to extracts from young and mature mango (Magnifera indica) leaves and stem-bark of the same plant. Study Design: The study employed statistical analysis of the data and interpretation. Place and Duration of Study: Young and mature mango leaves and stem-barks were collected from the Botanical Garden, Kenule Beeson Saro-Wiwa Polytechnic, Bori, Nigeria, and taken to the laboratory for analyses. Methodology: The samples were dried in an oven at 80oC for 3 days. Thereafter, 50 g of each ground mango leaves and stem-bark (young and mature of the same plant) were soaked separately in 500 ml of water, ethanol (95% v/v), and acetic acid (99.9% v/v) for another 3 days. The soaked materials were filtered through Whatman No. 1 filter paper into sterile beakers and evaporated to dryness in a water-bath at 80oC. The dried extracts obtained were reconstituted with water at concentrations of 100, 75, 50 and 25 mg/ml. Test organisms, Candida albicans, Staphylococcus aureus and Escherichia coli were obtained after proper laboratory screening of isolates from the diagnostic laboratory of the Rivers State University Teaching Hospital, Port Harcourt, Nigeria, for confirmation of identity and storage in universal bottles in a refrigerator. Sensitivity tests were carried out with the agar well diffusion method against the test organisms, using tetracycline as standard control drug (for bacteria) and fluconazole (for Candida), with cultures incubated accordingly. The measured zones of inhibition were compared with the controls and interpreted as resistant, intermediate, or susceptible to mango extracts in accordance with the interpretive guidelines published by the National Committee for Clinical Laboratory Standards (NCCLS). Assays for minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were also carried out. Results: Results obtained showed that Escherichia coli was completely susceptible to acetic acid young leaf and young bark extracts at 100 mg/ml concentrations. Staphylococcus aureus was susceptible only to Acetic acid young leaf extract at 100 mg/ml. For Candida albicans complete susceptibility was with acetic acid young bark at 100 mg/ml. mature leaf extract (100 mg/ml ), acetic acid young bark extract (100 to 50 mg/ml ), aqueous young bark extract (100 mg/ml) and acetic acid mature Candida albicans was susceptible to acetic acid young and mature bark extract at 100 mg/ml concentration. Minimum inhibitory concentration (MIC) values of acetic acid young leaf extract for all three organisms were 12.5 mg/ml. MIC of ethanolic young leaf extract for E. coli was 12.5 mg/ml whereas that for C. albicans was 50 mg/ml. Minimum bacteriocidal concentration values were same as MIC. Conclusion: E. coli and S.aureus were found to be most susceptible to acetic acid young leaf and stem-bark mango extracts. For C. albicans susceptibility profiles were best with aceti acid young and mature stem-bark extracts. It was also found that mango phytochemicals have broad-spectrum antibacterial activity as well as antifungal properties. The study also reveals that young mango parts contain higher bioactive substances than mature parts. Finally, it was concluded that acetic acid extracts produced the highest antimicrobial effects whereas aqueous extracts produced the least.
Aim: This study was carried out to investigate the antibacterial properties and efficacy of mango (Mangifera indica) leaf extracts on some clinical isolates as test rganisms. Study Design: The study employed statistical analysis of the data and interpretation Place and Duration of Study: Young and mature mango leaves were collected from the Botanical Garden, Kenule Beeson Saro-Wiwa Polytechnic, Bori, Nigeria, and taken to the laboratory for analyses. Methodology: The samples were dried in an oven at 80oC for 3 days. Thereafter, 50 g of each ground mango leaf (young and mature leaves) were soaked separately in 500 ml of water, ethanol (95% v/v), and acetic acid (99.9% v/v) respectively for another 3 days. The soaked materials were filtered through Whatman No. 1 filter paper into sterile beakers and evaporated to dryness in a water bath at 80oC. The dried extracts obtained were reconstituted with water at concentrations of 100, 75, 50, and 25 mg/ml. Test organisms, Escherichia coli, Staphylococcus aureus, Salmonella typhi, Proteus mirabilis, Bacillus cereus, and Pseudomonas aeruginosa were obtained after proper laboratory screening of isolates from the diagnostic laboratory of the Rivers State University Teaching Hospital, Port Harcourt, Nigeria, for confirmation of identity and storage in universal bottles in a refrigerator. Sensitivity tests were carried out with the agar well diffusion method against the test organisms, using tetracycline as the standard control drug, with cultures incubated accordingly. The measured zones of inhibition were compared with the controls and interpreted as resistant, intermediate, or susceptible to mango extracts in accordance with the interpretive guidelines published by the National Committee for Clinical Laboratory Standards (NCCLS). Assay for minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) was also carried out. Results: Results obtained showed that acetic acid young leaf extract at 100mg/ml produced 50 % susceptibility and 50 % intermediate response of test bacterial species. Generally, at 100 mg/ml, acetic acid young leaf extracts yielded 50% susceptibility and 50% intermediate response among both Gram-positive and Gram-negative bacteria. Ethanolic extracts gave 100% intermediate sensitivity of Gram-negative species and 50% each of resistant and intermediate response in Gram-positive forms. Aqueous extracts also produced no susceptibility among the test organisms as there was 100% resistance. Extracts of mature mango leaves of all solvents and at all concentrations used yielded no susceptibility response among the test bacterial species on the NCCLS scale. Minimum inhibitory and bactericidal concentrations were found to range from 25 mg/ml to 50 mg/ml. Additionally, it was observed that the sensitivity of organisms to mango extracts increased with concentration. Conclusion: In conclusion, acetic acid has a better extracting potential than ethanol and water as a solvent for the extraction of mango parts. More so, young mango leaves extracted with acetic acid possess higher broad-spectrum antibacterial properties than the mature mango leaves extracted from the same plant. It is therefore recommended that young mango leaves, extracted with acetic acid, be used for the treatment of microbial infections at concentrations not below 50 mg/ml.
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