We report a simple and effective approach to develop an electrochemical biosensor based on molecular-imprinted polymer to detect chloramphenicol (CAP). Polyaniline nanowires (PANi NWs) were grown on Au working electrode using cyclic voltammetry (CV) and chronoamperometry (CA). Using CV analysis, several kinetic parameters of interest, such as diffusion coefficient, D, and the heterogeneous standard rate constant, k 0 , were determined. The formation of PANi in the electrode occurred through a diffusion-controlled process with slow kinetics. The PANi NWs fabricated with CA were smoother, more homogenous, and showed less agglomeration than those fabricated using CV. CAP was imprinted into the PANi NW network through copolymerization using CA. CA was used to remove CAP from the PANi NW network in H 2 SO 4 0.5 M solution. The sensor can detect CAP with a limit of 1.24 × 10 −9 M, which is effective for monitoring CAP concentration in aquaculture.
Molecular imprinted polymer (MIP) nanowires were deposited on a gold electrode by electro-polymerization using aniline monomer as reagent and chloramphenicol (CAP) as template molecules. The molecular template was then electrochemically removed to generate the cavities on the polyaniline matrix that enables a specific recognition of CAP. The morphology, structure and electrochemical properties of the MIP were characterized by SEM, FT-IR and cyclic voltammetry. The initial results show that the MIP sensor can detect 10 -7 mM of CAP and the limit of detection could be lower by performing recommended study.
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