Molecularly Imprinted Polyaniline Nanowire-Based Electrochemical Biosensor for Chloramphenicol Detection: A Kinetic Study of Aniline Electropolymerization

Abstract

We report a simple and effective approach to develop an electrochemical biosensor based on molecular-imprinted polymer to detect chloramphenicol (CAP). Polyaniline nanowires (PANi NWs) were grown on Au working electrode using cyclic voltammetry (CV) and chronoamperometry (CA). Using CV analysis, several kinetic parameters of interest, such as diffusion coefficient, D, and the heterogeneous standard rate constant, k 0 , were determined. The formation of PANi in the electrode occurred through a diffusion-controlled process with slow kinetics. The PANi NWs fabricated with CA were smoother, more homogenous, and showed less agglomeration than those fabricated using CV. CAP was imprinted into the PANi NW network through copolymerization using CA. CA was used to remove CAP from the PANi NW network in H 2 SO 4 0.5 M solution. The sensor can detect CAP with a limit of 1.24 × 10 −9 M, which is effective for monitoring CAP concentration in aquaculture.

Metal-Organic Framework MIL-53(Fe): Synthesis, Electrochemical Characterization, and Application in Development of a Novel and Sensitive Electrochemical Sensor for Detection of Cadmium Ions in Aqueous Solutions

“…In the cases of both anodic and cathodic peaks, the peak currents expressed linear dependence on the square root of the potential scan rate, indicating that the electrochemical processes occurred in a diffusion-controlled regime on the MIL-53(Fe)-modified GCE electrode [40]. Besides, the relationships between the potentials of anodic and cathodic peaks and ln ν were investigated, and their corresponding linear fit lines for each set of experimental data have been shown in Figure 4(c), i.e., E pa vs. ln ν (the green line with the square symbols) y = 0:0178 × ln ν + 0:3099 R 2 = 0:9940…”

“…Figure 4: (a) Cyclic voltammograms of the GCE/MIL-53(Fe) electrode in 0.1 M KCl solution containing 5 mM Fe(CN) 63-/4at different scan rates of25,30,35,40,45,50, 55, 60, 65, 70, and 75 mV s -1 ; (b) the relationships between the currents of anodic and cathodic peaks and the square root of the scan rate (ν 1/2 ) and their corresponding linear fit lines: I pa , the red line with the triangle symbols, and I pc , the blue line with the circle symbols; (c) the relationships between the potentials of anodic and cathodic peaks and ln ν and their corresponding linear fit lines: E pa , the green line with the square symbols, and E pc , the orange line with the star symbols.…”

Electrosynthesis of electrochemically reduced graphene oxide/polyaniline nanowire/silver nanoflower nanocomposite for development of a highly sensitive electrochemical DNA sensor

“… 8 The existence of PANi in various oxidation states and protonation degree ranging from the most reduced leucoemeraldine form, through the half-oxidized emeraldine base form to the fully oxidized pernigraniline form, makes it an interesting material. 9 As a result, nanostructured PANi has a large variety of applications including sensors, nano-electronic devices, catalysts, electron field emitters, actuators, membranes, supercapacitors and batteries. 10–14 In the field of electrochemical biosensors, PANi is commonly used due to its abilities to act as a linking agent for the immobilization of biomolecules on the electrode surface, and to enhance the efficiency of electron transfer between the transducer and the electrode surface.…”

A New Nanomaterial Based Biosensor for MUC1 Biomarker Detection in Early Diagnosis, Tumor Progression and Treatment of Cancer

“…The most commonly used conductive polymers are polyaniline, polypyrrole, polythiophene and poly (3,4-ethylenedioxythiophene) in the design of biosensors [78,79]. For example, in order to detect some biomolecules, Ramanaviciene et al [82].…”