Polygonati Rhizoma (Huangjing) is traditional medicine in China, which can only be used as medicine after being processed. However, there is a limited theoretical basis for analyzing the changes in chemical components after traditional processing. In this study, analytical techniques including Fourier transform infrared spectroscopy, highperformance gel permeation chromatography-evaporative light scattering detection, and HPLC-diode array detection were proposed to perform multiple fingerprint analyses of the changes in the processed materials; the total sugar was also determined.Moreover, the chemometric studies, including hierarchical cluster analysis and principal component analysis, were used to visualize the discrimination of raw and processed materials. The results revealed that the chemical constituents had been profoundly changed following sample processing. In conclusion, these methods could be successfully used to compare raw and processed materials of Polygonatum kingianum, which could be used to elaborate the rationality of processing from the perspective of chemical composition.
Gentiana rigescens Franch. ex Hemsl. (Gentianaceae) is an endangered medicinal plant endemic to the southwest of China. The chloroplast (cp) genome of G. rigescens was 146,891 bp in length, with a large single-copy (LSC) region of 79,377 bp, a small single-copy (SSC) region of 17,026 bp, and a pair of inverted repeats (IRs) of 25,244 bp, forming a typical quadripartite structure. A total of 130 genes were annotated from the cp genome of G. rigescens, including 85 protein-coding genes, 37 tRNA genes, and 8 ribosomal RNA genes. Phylogenetic analysis indicated that G. rigescens was closely related to G. tonggolensis with strong bootstrap values belonging to the subfamily Gentianoideae.
Paris polyphylla
var.
alba
is a medicinal plant commonly used in the southwest of China. This study characterized the complete chloroplast (cp) genome sequence of
P. polyphylla
var.
alba
to investigate its phylogenetic relationship in Melanthiaceae. The cp genome of
P. polyphylla
var.
alba
is 165,079 base pairs (bp) in length with 36.96% G + C content. The cp genome is divided into (a) large single copy (LSC) (84,393 bp), (b) small single copy (SSC) (16,066 bp), and (c) two inverted regions (32,310 bp). The cp genome contains 135 genes, including 89 protein-coding genes, 38 tRNA genes, and 8 rRNA genes. Phylogenetic analysis indicated that
P. polyphylla
var.
alba
is closest to
P. polyphylla
var.
emeiensis
, and
Paris
had a close relationship with
Trillium
in Melanthiaceae.
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