SUMMARYThe phylogenetic and epidemic relationships of 104 clinical isolatesof Clostridium difficilefrom three hospitals of different geographical and population sources in China were investigated by multilocus sequence typing. Twenty-two sequence types (STs) were identified, four of which, ST117, ST118, ST119 and ST129, were novel. No geographically specific and host population-specific phylogenetic lineages were found and there was no correlation between geographical origin or host population and strain genotype. ST37 was the dominant type in our survey but the four novel STs underline the high genetic diversity and unique polymorphisms inC. difficilefrom China.
Based on the principle of vacuum counter-pressure casting, a low gas pressure infiltration technology was developed to fabricate the Ni-coated carbon fiber reinforced A357 alloy composites. The soundness and microstructure of the as-cast composites were investigated. The results show the relative density increases with the increase of melt temperature, while it firstly increases and then declines as the fiber temperature and infiltration pressure increased. The enhancement of melt and fiber temperature can eliminate the incomplete infiltration defects and improve the uniformity of fiber distribution. The insufficient infiltration pressure leads to some micro-pores in the matrix alloy. However, the over high fiber temperature and infiltration pressure may result in the separation of nickel coating and the fiber aggregation respectively, both of which are responsible for the partial un-infiltrated or insufficient filling defects. The appropriate infiltration parameters identified in this study could provide a reference for inhibition of the hazard interfacial reactions by optimizing the low gas pressure infiltration process.
During vacuum counter-pressure casting, the vacuumization is an important stage for thin-wall and complicated aluminum alloy castings. Through testing and an analyzing numbers of pinhole and pinhole grade of aluminum alloy samples under different vacuum degree, the effect of vacuum degree on pinhole of vacuum counter- pressure casting molten aluminum alloy is studied. The results indicate vacuum degree has greater effect on pinhole of molten aluminum alloy in vacuumizing process. The smaller vacuum degree is, the more numbers of pinhole of the samples are, and the higher pinhole grade of the samples is. Therefore, when vacuum degree is small, pinhole of molten aluminum alloy appears easily. So the vacuum degree doesn’t choose too small, but for thin-wall and complicated aluminum alloy castings, the vacuum degree doesn’t choose too big either. Appropriate vacuum degree is from 20 KPa to 40 KPa.
The solidification pressure is one of the most important factors in the counter-gravity casting process. Through testing and analyzing the microstructures of aluminum alloy castings under different solidification pressure, the effect of different solidification pressure on the secondary dendrite arm spacing and grain size is studied. The results show that with the increase of the solidification pressure, the secondary dendrite arm spacing and the grain size of aluminum alloy decreases. When the solidification pressure is 250 KPa, the secondary dendrite arm of aluminum alloy is thick, the SDAS is 37.9μm, but when the solidification pressure increases to 450 KPa, the refinement of grain is obviously, and the SDAS is 20.7μm, which is reduced by 45.3% comparing to solidification under 250 KPa. Moreover, when solidification pressure higher, the effect of feeding force becomes more evident, and the dendrite is broken when the feeding force higher than the strength of dendrite. Therefore, the grain size becomes more and more uniform and thin, and the (SDAS) of aluminum alloy are more and more small.
The SLS (Selective Laser Sintering, SLS) technology can directly manufacturing any complex structure, however, due to the limitation of the manufacturing engineering factor, the surface quality and the dimension accuracy of the green part are still an outstanding problem. So, the experimental methods were adopted to study the effect of part orientation on the machining accuracy, the part orientation will significantly affects the outline of the part due to the stair-stepping phenomenon, and then affects the surface quality and the dimension accuracy. The experimental samples of different part orientations including 0, 20, 45, 60, 90 degree were fabricated, and the results indicated that the stair-stepping phenomenon significantly affected by the part orientation, when the part orientation is 0, 45 and 90 degree, the dimension accuracy is best than other part orientation, it mean the stair-stepping phenomenon is smallest. So, when the part structure is complicated, the important structure dimension should be chosen the orientation 0, 45, or 90 degree
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