Pretreated
sugar cane bagasse (SCB) could be directly degraded
by a designed coculture system with Clostridium thermocellum and Thermoanaerobacterium aotearoense for biological
hydrogen and ethanol production, and the production was remarkably
improved by CaCO3 supplementation. Here, the effects of
CaCO3 concentration (10–100 mM) on the production
of hydrogen and ethanol were investigated. Under the optimal CaCO3 concentration of 40 mM, hydrogen production reached 87.56
± 4.08 mmol/L from 2% pretreated SCB with a yield of 4.38 mmol
H2/g SCB, an 88.62% increase over the culture without added
CaCO3 (46.42 ± 1.22 mmol/L and 2.32 mmol H2/g SCB). Additionally, the maximum ethanol concentration reached
10.60 ± 0.81 mM, a 192.82% increase over the control (3.62 ±
0.14 mM). The stimulatory effect of CaCO3 on the biodegradation
of SCB was primarily ascribed to the buffering capacity of CO3
2–.This study developed a novel strategy
to improve SCB biodegradation for biofuel production.
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