Enhanced biohydrogen production from sugarcane bagasse by Clostridium thermocellum supplemented with CaCO 3

Tian, Qing-Qing; Liu, Liang; Zhu, Ming‐Jun

doi:10.1016/j.biortech.2015.08.111

Cited by 67 publications

(19 citation statements)

References 30 publications

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“…However, further studies on real lignocellulosic substrates are essential to determine its relative importance as a potential CBP candidate for ethanol production. So far, single-step ethanol production involving different C. thermocellum strains has been attempted on a variety of complex lignocellulosic material such as corn stalk [57], corn cobs [58], corn stover [36], switchgrass [37, 59, 60], Populus [35, 61, 62], sugarcane bagasse [63–66], and wheat straw [67]. However, in order to investigate this ability for strain DBT-IOC-C19 and DSM 1313, rice straw biomass in native and pretreated forms was used and their fermentation performance was compared.…”

Section: Resultsmentioning

confidence: 99%

Cellulosic ethanol production via consolidated bioprocessing by a novel thermophilic anaerobic bacterium isolated from a Himalayan hot spring

Singh

Mathur

Tuli

et al. 2017

Biotechnol Biofuels

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BackgroundCellulose-degrading thermophilic anaerobic bacterium as a suitable host for consolidated bioprocessing (CBP) has been proposed as an economically suited platform for the production of second-generation biofuels. To recognize the overall objective of CBP, fermentation using co-culture of different cellulolytic and sugar-fermenting thermophilic anaerobic bacteria has been widely studied as an approach to achieving improved ethanol production. We assessed monoculture and co-culture fermentation of novel thermophilic anaerobic bacterium for ethanol production from real substrates under controlled conditions.ResultsIn this study, Clostridium sp. DBT-IOC-C19, a cellulose-degrading thermophilic anaerobic bacterium, was isolated from the cellulolytic enrichment cultures obtained from a Himalayan hot spring. Strain DBT-IOC-C19 exhibited a broad substrate spectrum and presented single-step conversion of various cellulosic and hemicellulosic substrates to ethanol, acetate, and lactate with ethanol being the major fermentation product. Additionally, the effect of varying cellulose concentrations on the fermentation performance of the strain was studied, indicating a maximum cellulose utilization ability of 10 g L−1 cellulose. Avicel degradation kinetics of the strain DBT-IOC-C19 displayed 94.6% degradation at 5 g L−1 and 82.74% degradation at 10 g L−1 avicel concentration within 96 h of fermentation. In a comparative study with Clostridium thermocellum DSM 1313, the ethanol and total product concentrations were higher by the newly isolated strain on pretreated rice straw at an equivalent substrate loading. Three different co-culture combinations were used on various substrates that presented two-fold yield improvement than the monoculture during batch fermentation.ConclusionsThis study demonstrated the direct fermentation ability of the novel thermophilic anaerobic bacteria on various cellulosic and hemicellulosic substrates into ethanol without the aid of any exogenous enzymes, representing CBP-based fermentation approach. Here, the broad substrate utilization spectrum of isolated cellulolytic thermophilic anaerobic bacterium was shown to be of potential utility. We demonstrated that the co-culture strategy involving novel strains is efficient in improving ethanol production from real substrate.Electronic supplementary materialThe online version of this article (doi:10.1186/s13068-017-0756-6) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Cellulosic ethanol production via consolidated bioprocessing by a novel thermophilic anaerobic bacterium isolated from a Himalayan hot spring

Singh

Mathur

Tuli

et al. 2017

Biotechnol Biofuels

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“…Furthermore, co-culture of C. thermocellum and Thermoanaerobacterium aotearoense can enhance the hydrogen production [1]. Besides, our studies showed that surfactant PEG8000 and CaCO3 both have positive effects on the hydrogen production by C. thermocellum [2][3][4].…”

mentioning

confidence: 63%

Biohydrogen Production from Waste Biomass

Zhu

2016

Tr Ren Energy

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“…Tian et al. have found that the biohydrogen production from sugarcane bagasse by C. thermocellum could be enhanced by CaCO 3 , and Bu et al. also found a similar phenomenon in a co‐culture system.…”

Section: The Application Of Cellulosome In Biorefinerymentioning

confidence: 83%

Reconstitution of cellulosome: Research progress and its application in biorefinery

Zhu

2019

Biotech and App Biochem

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Lignocellulose, one of the most abundant renewable sources of sugar, can be converted into bioenergy through hydrolysis of cellulose and hemicellulose. Due to its renewability and availability in large quantities, bioenergy is considered as a possible alternative to fossil energy and attracts the attention of the world with increased concerns about environmental protection and energy crisis. The depolymerization of cellulosic substrate to monomer is the rate‐limiting step in the bioconversion of lignocellulose by cellulolytic microbes. Cellulosome, a multienzyme complex from anaerobic cellulolytic bacteria, can efficiently degrade the cellulosic substrates. Previous studies have shown that the reconstitution of cellulosome in vitro and its heterologous expression or display on the cell surface can help to solve the low yield problem of cellulosome in cellulolytic bacteria. This paper reviews the research progress in the reconstitution of cellulosome as well as its application in biorefinery, including the construction of cellulosome as well as different methods for cellulosome reconstitution and its surface display. This review will promote the understanding of cellulosome and its reconstitution.

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Enhanced biohydrogen production from sugarcane bagasse by Clostridium thermocellum supplemented with CaCO 3

Cited by 67 publications

References 30 publications

Cellulosic ethanol production via consolidated bioprocessing by a novel thermophilic anaerobic bacterium isolated from a Himalayan hot spring

Cellulosic ethanol production via consolidated bioprocessing by a novel thermophilic anaerobic bacterium isolated from a Himalayan hot spring

Biohydrogen Production from Waste Biomass

Reconstitution of cellulosome: Research progress and its application in biorefinery

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