We mainly studied the mixing performance of three new T-type electroosmotic micromixers (T-EMs). The three T-EMs are designed as rhomboids with three different shapes of obstructions inside. The rhomboid micromixers utilize the principle of convergence and divergence to improve mixing efficiency. By changing the Reynolds number (Re), the change of mixing efficiency under direct current (DC) voltage 20[Formula: see text]V and alternating current (AC) voltage 2[Formula: see text]mV was observed. We changed the shape of the intermediate obstacles, discussed the shape of the internal barrier on the mixing efficiency of T-EM, and increased the mixing efficiency by increasing the voltage value. The mixing efficiency of T-EM with 150[Formula: see text][Formula: see text]m channel width can reach 97.5% under DC condition and 97.6% with circular barrier under AC condition.
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