Insights into a deterministic lateral displacement sorting chip with new cross-section micropillars

Chen, Xueye; Feng, Qiaoyu; Zhang, Yaolong

doi:10.1016/j.chaos.2022.111884

Cited by 7 publications

(3 citation statements)

References 28 publications

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“…Unlike traditional micropillar arrays, which commonly act as a filter and only allow small objects to pass through while blocking large ones, DLD micropillar arrays allow objects of various sizes to pass. [ 107 ] In DLD‐based cell sorting, cells/particles with radii smaller than a critical radius pass through the intercolumn gaps and move along the main flow direction. Cells/particles with radii larger than the critical radius migrate laterally to adjacent streamlines determined by the shape and the geometry of the micropillar array ( Figure a).…”

Section: Methods For Cell Sorting and Isolation In Integrated Microfl...mentioning

confidence: 99%

Integrated Microfluidics for Single‐Cell Separation and On‐Chip Analysis: Novel Applications and Recent Advances

Kutluk,

Viefhues,

Constantinou

2024

Small Science

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From deciphering infection and disease mechanisms to identifying novel biomarkers and personalizing treatments, the characteristics of individual cells can provide significant insights into a variety of biological processes and facilitate decision‐making in biomedical environments. Conventional single‐cell analysis methods are limited in terms of cost, contamination risks, sample volumes, analysis times, throughput, sensitivity, and selectivity. Although microfluidic approaches have been suggested as a low‐cost, information‐rich, and high‐throughput alternative to conventional single‐cell isolation and analysis methods, limitations such as necessary off‐chip sample pre‐ and post‐processing as well as systems designed for individual workflows have restricted their applications. In this review, a comprehensive overview of recent advances in integrated microfluidics for single‐cell isolation and on‐chip analysis in three prominent application domains are provided: investigation of somatic cells (particularly cancer and immune cells), stem cells, and microorganisms. Also, the use of conventional cell separation methods (e.g., dielectrophoresis) in unconventional or novel ways, which can advance the integration of multiple workflows in microfluidic systems, is discussed. Finally, a critical discussion related to current limitations of integrated microfluidic single‐cell workflows and how they could be overcome is provided.

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Section: Methods For Cell Sorting and Isolation In Integrated Microfl...mentioning

confidence: 99%

Integrated Microfluidics for Single‐Cell Separation and On‐Chip Analysis: Novel Applications and Recent Advances

Kutluk,

Viefhues,

Constantinou

2024

Small Science

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“…The taSSAW microfluidic has been widely applied to biological particle separation, such as bacteria [ 28 ], tumor cells [ 26 , 27 ], extracellular microvesicles [ 29 ], and exosomes [ 17 , 30 ]. To fully grasp the potential of taSSAW microfluidic devices, further analysis of particle deflection and development in the reliability of the available devices is mandatory, which can be achieved by a numerical and analytical study [ [31] , [32] , [33] ]. Numerical methods have been demonstrated for the reliable design of bioparticle separation microfluidic devices [ [34] , [35] , [36] , [37] , [38] ].…”

Section: Introductionmentioning

confidence: 99%

Investigation on submicron particle separation and deflection using tilted-angle standing surface acoustic wave microfluidics

Peng,

Lin,

et al. 2024

Heliyon

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“…The TEER electrode location effects the changes in TEER values in cultures [14][15][16]. Microfluidic chip design and simulations are also playing important role in symmetry of the cell and velocity of the fluids in microfluidic systems [17]. Microfluidic systems with embedded sensors are increasingly being developed owing to their rapid efficacy testing capacity; for example, strain sensors and humidity sensors for health tracking and production of implantable applications [18].…”

Section: Introductionmentioning

confidence: 99%

Biological Influence of Pulmonary Disease Conditions Induced by Particulate Matter on Microfluidic Lung Chips

Jabbar

Kim²,

Lee

2022

BioChip J

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Particulate matter (PM10)-induced respiratory illnesses are difficult to investigate in trans-well culture systems. Microphysiological systems offer the capacity to mimic these phenomena to analyze any possible hazards that PM10 exposure poses to respiratory system of Humans. This study proposes an on-chip healthy human lung distal airway model that efficiently reconstitutes in vivo-like environmental conditions in a microfluidic device. The lung-on-chip model comprises a TEER sensor chip and portable microscope for continuous monitoring. To determine the efficacy of our model, we assessed the response to exposure to three PM environmental conditions (mild, average, and severe) and analyzed the relevant in vivo physiological and toxicological data using the airway model. Our results revealed significant increases in the levels of the IL-13, IL-6, and MUC5AC pathological biomarkers, which indicate increased incidences of on-chip asthma and chronic obstructive pulmonary disease conditions. Overall, we deduced that this model will facilitate the identification of potential therapeutics and the prevention of chronic life-threatening toxicities and pandemics such as COVID-19. The proposed system provides basic data for producing an improved in organ-on-chip technology.

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Insights into a deterministic lateral displacement sorting chip with new cross-section micropillars

Cited by 7 publications

References 28 publications

Integrated Microfluidics for Single‐Cell Separation and On‐Chip Analysis: Novel Applications and Recent Advances

Integrated Microfluidics for Single‐Cell Separation and On‐Chip Analysis: Novel Applications and Recent Advances

Investigation on submicron particle separation and deflection using tilted-angle standing surface acoustic wave microfluidics

Biological Influence of Pulmonary Disease Conditions Induced by Particulate Matter on Microfluidic Lung Chips

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