Background Globally, the prevalence of anemia among women of reproductive age is about 29.4%, and anemia impacts about 40% of pregnant women and more than 20% of non-pregnant women. We conducted a longitudinal observational study of anemia in pregnant and non-pregnant women, and analyzed the association between the prevalence of anemia and sociodemographic characteristics of women in southwest China. Methods This study was a longitudinal observational study which involved 640,672 women aged 18–49 years from 129 counties in southwest China. Data were from databases of National Free Preconception Health Examination Project (NFPHEP) and electronic medical records of local hospitals. We adjusted the diagnostic thresholds of anemia for altitude. The prevalence of anemia was expressed in percentages and 95% confidence intervals (95% CI). The association between the prevalence of anemia and sociodemographic characteristics of pregnant and non-pregnant women were analyzed using univariate and multivariate logistic regression method, expressed in crude odds ratio (cOR), adjusted odds ratio (aOR) and 95%CI. Results Of the 640,672 participants, 121,254 women suffered from anemia, with the prevalence of 18.9% (95%CI: 18.8–19.0%). From 2014 to 2018, the prevalence of anemia declines from 23.0–16.4%.The prevalence was 21.6% in the first trimester, higher than women in non-pregnancy (17.4%) and women in the third trimester (10.5%). Results from the multivariable logistic regression showed that women aged 18–20 (aOR = 1.28) or over 35 years old (aOR = 1.07), being farmers (aOR = 1.42), being ethnic minorities (aOR: 1.19 ~ 1.73), during the first trimester (aOR = 1.32) were more likely to be anemic. Conclusions Although the anemia prevalence of women of reproductive age has been decreasing in recent years, the prevalence of anemia is still high in pregnant and non-pregnant women in southwest China, especially during the first trimester. Women who were older or younger, being farmers, being ethnic minorities were at high risk of anemia. Anemia in women of reproductive age cannot be neglected.

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To make environmental management decisions more executive and targeted, it is essential for decision-making to include local residents’ perceptions and preferences for ecosystem services (ES) and biodiversity (BD). This study conducted a questionnaire survey with 386 local residents to explore social perceptions for ES and BD in the Fenghe River watershed. ES contain food from agriculture (AGR), food from livestock (LIV), fresh water (FW), air purification (AP), water purification (WP), water retention (WR), soil retention (SR), aesthetics (AES), recreation (RE), and spirit (SP) in this study. Ranking and Likert scales are combined to identify residents’ preferences for ES and BD. The hypothetical market method is used to identify the willingness to pay for BD and ES. Independent sample T-test, one-way ANOVA, and Spearman correlation are used to analyze preference heterogeneity. The results show that: (1) residents prefer WP, AP, AGR, and FW; (2) 51.3% of respondents are willing to pay a fee for improving ES while 48.7% of respondents are unwilling to pay; (3) residents’ personal and residential factors affect preference heterogeneity. Last, we put forward three management suggestions on controlling environmental pollution, improving residents’ awareness of ES, and establishing a multi-channel fund based on government financial resources for improving ES in the Fenghe River watershed. Integrating stakeholders’ perceptions for ES into decision-making can promote the sustainable development in Fenghe River watershed.

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Sichuan, China, has abundant genetic resources of sheep (Ovis aries). However, their genetic diversity and population structure have been less studied, especially at the genome-wide level. In the present study, we employed the specific-locus amplified fragment sequencing for identifying genome-wide single nucleotide polymorphisms (SNPs) among five breeds of sheep distributed in Sichuan, including three local pure breeds, one composite breed, and one exotic breed of White Suffolk. From 494 million clean paired-end reads, we obtained a total of 327,845 high-quality SNPs that were evenly distributed among all 27 chromosomes, with a transition/transversion ratio of 2.56. Based on this SNP panel, we found that the overall nucleotide diversity was 0.2284 for all five breeds, with the highest and lowest diversity observed in Mage sheep (0.2125) and Butuo Black (0.1963) sheep, respectively. Both Wright’s fixation index and Identity-by-State distance revealed that all individuals of Liangshan Semifine-wool, White Suffolk, and Butuo Black sheep were respectively clustered together, and the breeds could be separated from each other, whereas Jialuo and Mage sheep had the closest genetic relationship and could not be distinguished from each other. In conclusion, we provide a reference panel of genome-wide and high-quality SNPs in five sheep breeds in Sichuan, by which their genetic diversity and population structures were investigated.

Urban areas are the areas that are most strongly affected by human activities, which presents many challenges to the ecosystem and human well-being. Ecosystem services (ES) are a comprehensive indicator to measure the ecological effects of urbanization. To effectively identify and evaluate the impact of urbanization on ES, the spatial-temporal pattern of ES should be considered. According to the level of urbanization, Xi’an city is divided into four regions: the urban core area, the urban extended area, the rural area, and the ecological conservation area, then, five comprehensive ecosystem services (CES) are evaluated by In VEST model. The results showed the following: (1) There is an obvious spatial heterogeneity in the distribution of ES. The ecological conservation area is the hot spot of ES supply, and the low value is mostly distributed in the urban core area. (2) The CES in the urban extended area that has undergone the greatest change between 2000 and 2015, and the rates of change in the ecological conservation area are the smallest. (3) There is a significant correlation between urbanization and ES, and the correction between landscape urbanization and ES is the most significant. (4) The agglomeration relationship between urbanization and ES in different regions is not consistent. Regional division provides a new way to understand the interaction between urbanization and ES in time and space, so as to provide better guidance for policy makers in formulating sustainable development policies to alleviate the loss of ES caused by the process of urbanization.