No abstract

No abstract

Background Oryza glumaepatula represents an important resource of genetic diversity that can be used to improve rice production. However, hybrid sterility severely restricts gene flow between Oryza species, and hinders the utilization of distant heterosis in hybrid rice breeding. Results In order to fully exploit the beneficial genes of O. glumaepatula and facilitate the conservation of these gene resources, a set of chromosome single-segment substitution lines (SSSLs) was developed using an indica variety HJX74 as the recurrent parent and an accession of O. glumaepatula as the donor parent. During the process of SSSLs development, S23 , a locus conferring hybrid male sterility between O. sativa and O. glumaepatula , was identified and fine mapped to 11.54 kb and 7.08 kb genomic region in O. sativa and O. glumaepatula , respectively, encoding three and two candidate ORFs, respectively. qRT-PCR and sequence analysis excluded one common ORF as the candidate gene. In addition, hybrid male sterility caused by S23 was environment-sensitive, and could be observed only in natural short-day (NSD). Conclusion Identification and candidate genes analysis of S23 in this study provides a valuable example to study the crosstalk between interspecific F 1 hybrid male sterility and environment-conditioned male sterility in rice, facilitates reserving and utilizing favorable genes or alleles of wild Oryza species, and allows for a more efficient exploitation of distant heterosis in hybrid rice breeding. Electronic supplementary material The online version of this article (10.1186/s12284-019-0271-4) contains supplementary material, which is available to authorized users.

Recurrent pregnancy loss (RPL) is a common reproductive problem affecting around 5% of couples worldwide. At present, about half of RPL cases remained unexplained. Previous studies have suggested an important role for genetic determinants in the etiology of RPL. Here, we performed whole-exome sequencing (WES) analysis on 100 unrelated Han Chinese women with a history of two or more spontaneous abortions. We identified 6736 rare deleterious nonsynonymous variants across all patients. To focus on possible candidate genes, we generated a list of 95 highly relevant genes that were functionally associated with miscarriage according to human and mouse model studies, and found 35 heterozygous variants of 28 RPL-associated genes in 32 patients. Four genes (FOXA2, FGA, F13A1, and KHDC3L) were identified as being strong candidates. The FOXA2 nonsense variant was for the first time reported here in women with RPL. FOXA2 knockdown in HEK-293T cells significantly diminished the mRNA and protein expression levels of LIF, a pivotal factor for maternal receptivity and blastocyst implantation. The other genes, with 29 variants, were involved in angiogenesis, the immune response and inflammation, cell growth and proliferation, which are functionally important processes for implantation and pregnancy. Our study identified several potential causal genetic variants in women with RPL by WES, highlighting the important role of genes controlling coagulation, confirming the pathogenic role of KHDC3L and identifying FOXA2 as a newly identified causal gene in women with RPL.

No abstract

Losing an only child affects the mental health of many older adults in China, placing them at a higher risk of loneliness. However, few studies explore the long-term effects of losing an only child on loneliness trajectories among older Chinese bereaved parents. Adopting a qualitative, grounded theory approach, this study investigates how experiences of loneliness among bereaved parents evolve and the implications for staged intervention. Twenty-seven in-depth interviews were conducted with people who lost their only child in Shanghai and Wuhan. A multistep data analysis technique was used to analyze the data. The findings indicate that trajectories of perceived loneliness have three distinct stages and follow a U-shape in their frequency and intensity. Interventions of psychological consultation, social network restoration, and improved old-age support should be emphasized at each stage.

The exchange of information between animals is crucial for maintaining social relations, individual survival, and reproduction, etc. The uropygial gland is a particular secretion gland found in birds. We speculated that uropygial gland secretions might act as a chemical signal responsible for sexual communication. We employed non-targeted metabolomic technology through liquid chromatography and mass spectrometry (LC-MS) to identifying duck uropygial gland secretions. We observed 11,311 and 14,321 chemical substances in the uropygial gland secretion for positive and negative ion modes, respectively. Based on their relative contents, principal component analysis (PCA) showed that gender significantly affects the metabolite composition of the duck uropygial gland. A total of 3831 and 4510 differential metabolites were further identified between the two sexes at the positive and negative ion modes, respectively. Of them, 139 differential metabolites were finally annotated. Among the 80 differential metabolites that reached an extremely significant difference (p < 0.01), we identified 24 volatile substances. Moreover, we further demonstrated that five kinds of volatile substances are highly repeatable in all testing ducks, including picolinic acid, 3-Hydroxypicolinic acid, indoleacetaldehyde, 3-hydroxymethylglutaric acid, and 3-methyl-2-oxovaleric acid. All these substances are significantly higher in males than in females, and their functions are involved in the reproduction processes of birds. Our data implied that these volatile substances act as sex pheromones and may be crucial olfactory clues for mate selection between birds. Our findings laid the foundation for future research on whether uropygial gland secretion can affect ducks’ reproduction and production.

Objective: This study was conducted to clone and compare the molecular characteristics of the deiodinase 2 (DIO2) gene between Sichuan White geese and Landes geese, and to analyze the association between polymorphisms of the DIO2 gene and head dimensions in Tianfu meat geese.Methods: The coding sequence of the DIO2 gene was cloned by polymerase chain reaction and vector ligation and aligned by DNAMAN software. A total of 350 Tianfu meat geese were used to genotype the polymorphisms of the DIO2 gene and measure the head dimensions. Association analysis between the polymorphisms of the DIO2 gene and head dimensions was carried out.Results: An 840-bp coding sequence of the DIO2 gene was obtained and comparison analysis identified four polymorphic loci between Sichuan White geese and Landes geese. Further analysis showed that the dominant alleles for the four polymorphic loci were G, G, A, and T and the frequency of the heterozygous genotype was higher than that of the homozygous genotype in Tianfu meat geese. Compared to that in the population of non-knob geese of Tianfu meat geese, the head dimensions in the population of knob geese were significantly higher except for nostril height. However, in the non-knob geese, beak width 1, beak width 2, nostril length, cranial width 1, and maxillary length had significant differences among different genotypes or haplotypes/diplotypes.Conclusion: These results suggested that polymorphisms of the DIO2 gene could be considered molecular markers to select larger heads of geese in the population of non-knob geese.