Background Oryza glumaepatula represents an important resource of genetic diversity that can be used to improve rice production. However, hybrid sterility severely restricts gene flow between Oryza species, and hinders the utilization of distant heterosis in hybrid rice breeding. Results In order to fully exploit the beneficial genes of O. glumaepatula and facilitate the conservation of these gene resources, a set of chromosome single-segment substitution lines (SSSLs) was developed using an indica variety HJX74 as the recurrent parent and an accession of O. glumaepatula as the donor parent. During the process of SSSLs development, S23 , a locus conferring hybrid male sterility between O. sativa and O. glumaepatula , was identified and fine mapped to 11.54 kb and 7.08 kb genomic region in O. sativa and O. glumaepatula , respectively, encoding three and two candidate ORFs, respectively. qRT-PCR and sequence analysis excluded one common ORF as the candidate gene. In addition, hybrid male sterility caused by S23 was environment-sensitive, and could be observed only in natural short-day (NSD). Conclusion Identification and candidate genes analysis of S23 in this study provides a valuable example to study the crosstalk between interspecific F 1 hybrid male sterility and environment-conditioned male sterility in rice, facilitates reserving and utilizing favorable genes or alleles of wild Oryza species, and allows for a more efficient exploitation of distant heterosis in hybrid rice breeding. Electronic supplementary material The online version of this article (10.1186/s12284-019-0271-4) contains supplementary material, which is available to authorized users.

Background. Previous studies have shown that long noncoding RNAs (lncRNAs) play a key role in cancer, including colon cancer (CC). However, the exact role of long noncoding RNA 01124 (LINC01124) in CC and its mechanisms of action remain unknown. In this study, we investigated the functional effects and the possible mechanism of LINC01124 in CC. Methods. We first determined the expression of LINC01124 in CC tissues (The Cancer Genome Atlas (TCGA) database) and cell lines (quantitative real-time polymerase chain reaction (qRT-PCR)). Functional analysis via Cell Counting Kit-8 (CCK-8), colony formation, cell cycle, wound healing and Transwell assays were performed, and a mechanistic experiment was performed with the western blotting. The function of LINC01124 was also determined in vivo using nude BALB/c mice. Results. The results showed that LINC01124 was upregulated in CC tissues and cell lines. Functional studies showed that knockdown of LINC01124 significantly suppressed the proliferation, migration, and invasion of colon cancer cells in vitro and in vivo. Subsequent mechanistic experiments indicated that LINC01124 acted as a sponge to suppress microRNA 654-5p, which targeted HAX-1. Downregulation of LINC01124 decreased the expression of HAX-1, and overexpression of the miR-654-5p inhibitor attenuated the sh-LINC01124-induced inhibition of CC cell proliferation, migration, and invasion. Conclusion. Collectively, this study revealed that the knockdown of LINC01124 inhibited the malignant behaviors of CC via the miR-654-5p/HAX-1 axis, suggesting that LINC01124 might be a therapeutic target for CC treatment.

Objective The aim of this study was to investigate the mental health status and sleep quality of university students after local outbreak of COVID-19, and to help them understand the psychological stress reaction and provide base for their mental health education. Methods A cross-sectional survey was conducted on the mental health status of university students in a Wuhan-based university. Results A total of 897 university students were enrolled in the study. Compared with the epidemic period, university students' mental health status and sleep quality has a great deal of difference in the aspects of gender, grade, discipline and specialty, physical exercise, as well as with their family relationships and so on. 64.26% students would like to talk to their peers or close friends, while only 2.71% would like to call a caring hotline or seek help from a psychologist. Conclusion After the local outbreak of COVID-19 in Wuhan was contained, the mental health status and sleep quality of more than half of the students improved. However, priority attention and care should be given to female students, senior students, those undertaking literature and history majors and those dissatisfied with family relationships.

Background Previous studies have shown that long noncoding RNAs (lncRNAs) play a key role in cancer, including colon cancer (CC). However, the exact role of long noncoding RNA 01124 (LINC01124) in CC and its mechanisms of action remain unknown. In the present study, we aimed to investigate the functional effects and the possible mechanism of LINC01124 in CC. Results we demonstrated that LINC01124 was upregulated in colon cancer tissues and cell lines. Further functional studies showed that knockdown of LINC01124 significantly suppressed the proliferation, migration and invasion of colon cancer cells in vitro and in vivo. Moreover, in subsequent mechanistic experiments, LINC01124 was found to act as a sponge to suppress microRNA 654-5p, which targeted HAX-1. Downregulation of LINC01124 decreased the expression of HAX-1, and overexpression of a miR-654-5p inhibitor attenuated the sh-LINC01124-induced inhibition of CC cell proliferation, migration and invasion. Conclusion Collectively, all the results revealed a role for the LINC01124/miR-654-5p/HAX-1 axis in the tumorigenesis and progression of CC, suggesting that LINC01124 may be a therapeutic target for colon cancer treatment.