Microglia
as an important type of innate immune cell in the brain
have been considered as an effective therapeutic target for the treatment
of central nervous degenerative diseases. Herein, we report cell membrane
coated novel biomimetic Cu2–x
Se-PVP-Qe
nanoparticles (denoted as CSPQ@CM nanoparticles, where PVP is poly(vinylpyrrolidone),
Qe is quercetin, and CM is the cell membrane of neuron cells) for
effectively targeting and modulating microglia to treat Parkinson’s
disease (PD). The CSPQ nanoparticles exhibit multienzyme activities
and could effectively scavenge the reactive oxygen species and promote
the polarization of microglia into the anti-inflammatory M2-like phenotype
to relieve neuroinflammation. We reveal that biomimetic CSPQ@CM nanoparticles
targeted microglia through the specific interactions between the membrane
surface vascular cells adhering to molecule-1 and α4β1
integrin expressed by microglia. They could significantly improve
the symptoms of PD mice to result in an excellent therapeutic efficacy,
as evidenced by the recovery of their dopamine level in cerebrospinal
fluid, tyrosine hydroxylase, and ionized calcium binding adapter protein
1 to normal levels. Our work demonstrates the great potential of these
robust biomimetic nanoparticles in the targeted treatment of PD and
other central nervous degenerative diseases.
Objectives:
The goal of this study was to determine the role of microRNA transfer in mediating the effects of mesenchymal stem cell–derived extracellular vesicles in acute lung injury.
Design:
Experimental cell and animal studies.
Setting:
University-based research laboratory.
Subjects:
THP-1 monocytes, bone marrow–derived macrophages, and C57BL/6 mice.
Interventions:
To determine the microRNA transfer in vitro, mesenchymal stem cells and mesenchymal stem cell–derived extracellular vesicles were cultured with THP-1 cells and bone marrow–derived macrophages and then assayed for microRNA expression in the target cells. To examine the role of microRNA transfer in vivo, mesenchymal stem cell–derived extracellular vesicles were administered to mice with lipopolysaccharide-induced lung injury.
Measurements and Main Results:
Mesenchymal stem cell–derived extracellular vesicles were efficiently taken up by macrophages in vitro and in vivo. miR-27a-3p was one of the most highly expressed microRNAs in THP-1 cells in microarray analysis and was transferred from mesenchymal stem cells and mesenchymal stem cell–derived extracellular vesicles to THP-1/bone marrow–derived macrophages. Mesenchymal stem cell–derived extracellular vesicles promoted M2 polarization in bone marrow–derived macrophages, which was inhibited by lentiviral anti-miR-27a-3p transduction. Mesenchymal stem cell–derived extracellular vesicles administered systemically and intratracheally were as effective as mesenchymal stem cells in alleviating acute lung injury, elevating miR-27a-3p levels in alveolar macrophages, and promoting M2 macrophage polarization. Treatment of mesenchymal stem cell–derived extracellular vesicles concurrently decreased alveolar macrophage expression of nuclear factor kappa B subunit 1, a target of miR-27a-3p. Lentiviral transduction of mesenchymal stem cells with anti-miR-27a-3p or knockdown of miR-27a-3p in vivo abolished the effects of mesenchymal stem cell–derived extracellular vesicles on acute lung injury and M2 macrophage polarization.
Conclusions:
Mesenchymal stem cell–derived extracellular vesicles mitigate acute lung injury at least partially via transferring miR-27a-3p to alveolar macrophages. miR-27a-3p acts to target NFKB1 and is a crucial regulator of M2 macrophage polarization.
NÀ N Atropisomers are a common motif in natural products and represent a significant dimension for exploration in modern pharmaceutical and medicinal chemistry. However, the catalytic atroposelective synthesis of such molecules remains challenging, hampering meaningful development. In particular, an enantioselective synthesis of NÀ N bisindole atropisomers is unprecedented. Herein, the first enantioselective synthesis of NÀ N bisindole atropisomers via the palladium-catalyzed de novo construction of one indole skeleton is presented. A wide variety of NÀ N axially chiral bisindoles were generated in good yields with excellent enantioselectivities via a cascade condensation/N-arylation reaction. Structurally diverse indole-pyrrole, indolecarbazole, and non-biaryl-indole atropisomers possessing a chiral NÀ N axis were accessed using this protocol. Moreover, investigations using density functional theory (DFT) calculations provided insight into the reaction mechanism and enantiocontrol.
Nitrogen–nitrogen bonds containing
motifs are ubiquitous
in natural products and bioactive compounds. However, the atropisomerism
arising from a restricted rotation around an N–N bond is largely
overlooked. Here, we describe a method to access the first enantioselective
synthesis of N–N biaryl atropisomers via a Cu-bisoxazoline-catalyzed
Friedel–Crafts alkylation reaction. A wide range of axially
chiral N–N bisazaheterocycle compounds were efficiently prepared
in high yields with excellent enantioselectivities via desymmetrization
and kinetic resolution. Heating experiments showed that the axially
chiral bisazaheterocycle products have high rotational barriers.
N−C Biaryl atropisomers are prevalent in natural products and bioactive drug molecules. However, the enantioselective synthesis of such molecules has not developed significantly. Particularly, the enantioselective synthesis of N−C biaryl atropisomers by stereoselective metal‐catalyzed aryl amination remains unprecedented. Herein, a Pd‐catalyzed cross‐coupling strategy is presented for the synthesis of N−C axially chiral biaryl molecules. A broad spectrum of N−C axially chiral compounds was obtained with excellent enantioselectivities (up to 99 % ee) and good yields (up to 98 %). The practicality of this reaction was validated in the synthesis of useful biological molecules.
As a new-generation CDK inhibitor, a CDK4/6 inhibitor combined with endocrine therapy has been successful in the treatment of advanced estrogen receptor–positive (ER+) breast cancer. Although there has been overall progress in the treatment of cancer, drug resistance is an emerging cause for breast cancer–related death. Overcoming CDK4/6 resistance is an urgent problem. Overactivation of the cyclin-CDK-Rb axis related to uncontrolled cell proliferation is the main cause of CDK4/6 inhibitor resistance; however, the underlying mechanisms need to be clarified further. We review various resistance mechanisms of CDK4/6 inhibitors in luminal breast cancer. The cell signaling pathways involved in therapy resistance are divided into two groups: upstream response mechanisms and downstream bypass mechanisms. Finally, we discuss possible strategies to overcome CDK4/6 inhibitor resistance and identify novel resistance targets for future clinical application.
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