As an important metabolic intermediate of sulfur-containing
amino
acids in human body, homocysteine (Hcy) is regarded as an independent
risk factor for atherosclerotic cardiovascular disease. Therefore,
real-time monitoring of the fluctuation of Hcy level is of great importance
for the early diagnosis as well as the treatment of atherosclerosis.
Herein, a new two-photon (TP) fluorescent probe (RH-2) was developed via a hydrogen bond-assisted strategy, which had
a high specificity for detecting Hcy over cysteine (Cys) and glutathione
(GSH) in solution, cells, and tissue. Probe RH-2 was
applied to the quantitative determination of Hcy in human serum successfully.
Moreover, the two-photon fluorescence (TPF) imaging of abnormal expression
of Hcy in aortic vessels and liver of atherosclerotic model mice were
fulfilled by RH-2. Therefore, probe RH-2 can be served as a potential tool to understand the function of
Hcy in atherosclerosis, supplying a clinical promise for the early
diagnosis of atherosclerosis (AS).
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