a b s t r a c tThe morphological similarity among Sparidae species, which are characterized by a different market price, represents a serious problem for their trade and for stock management, since it encourages frauds for substitution. The most accredited morphological method for their identification is based on the dental-plate, but this approach is not simple and cannot be used for prepared products. When molecular methods are used the DNA degradation induced by cooking is the main drawback. In this work, we collected 314 reference tissues belonging to 75 Sparidae species and we produced a dataset of full (FDB) and mini-barcode (MDB) reference sequences starting from DNA extracted from fresh and ethanolpreserved tissues using universal primes. Moreover, some fresh samples were cooked. The FDB was successfully amplified in 91% (fresh), 50% (cooked) and 81% (ethanol-preserved) samples, while the amplification rates of the MDB were considerably higher in case of cooked (100%) and ethanol-preserved (94%) samples. The same primers were used for the amplification of the DNA obtained from 58 market samples (MS). All the DNA barcodes were compared with BOLD and GenBank using IDs and BLAST analysis. FDB was able to provide unambiguous species-level identifications for 53 (78%) and 44 (64.7%) reference samples analyzed on BOLD and GenBank, respectively. The Mini-DNA barcode (MDB) showed a lower discriminating power with 32 (45.7%) and 29 (41.4%) sequences unambiguously matched to a species on BOLD and GenBank. However, the MDB allowed to identify all the reference sequences as belonging to the Sparidae family. FDB and MDB showed a similar performance in analyzing the MS, allowing to highlight 21 (38%) mislabeled MS. Our study, while confirming the FDB as a reliable tool for fish authentication, proposes the MDB as a promising tool to recover molecular information in case of cooked products.
Exotic marine fish products are increasingly appreciated in China. In this study, 100 samples of Cod, Salmon and Tuna products were collected from supermarkets in Shanghai, Nanjing and Hangzhou. First the information reported on the label were assessed in the light of the Chinese legislation, paying particular attention to the fish names and the geographical origin. Then, a comparative analysis of the official trade denominations adopted by five European countries (Italy, France, Germany, Spain and United Kingdom) for Cod, Salmon and Tuna was performed. Finally, the Chinese names of the species considered in the EU list were verified consulting the available international lists. Overall, 95% of the samples employed just generic names. In particular, 98% of Salmon and 100% of Tuna products were generically labeled while the labeling of Cod products was more diversified, even though 80% reported misleading or fake denominations. The results of this work highlighted the lack of a mandatory legislation on seafood traceability and of an official naming system. In particular, this study propose the introduction of a detailed Chinese naming system based on the Chinese Latin Dictionary for Seafood Names, following the EU approach. In fact, inaccurate labeling can have both economic and health implications for consumers as well as it may distort the true abundance of fish stocks. These drawbacks can be particularly serious considering the pivotal role of China in the global fishery industry
The European Union (EU), within the renewal plan of the Common Fisheries Policy and the Common\ud Market Organization, with the Cape IV of Reg. (EU) n. 1379/2013 have introduced new requirements for\ud the labelling of fisheries and aquaculture products. These, as well as providing consumers with more\ud complete information, integrate the provisions of Reg. (EU) n. 1169/2011 and acts as a tool to prevent\ud frauds and illegal fishing. In this work the new seafood labelling provisions were evaluated, starting from\ud the analysis of the art. 35 of the Chapter IV and comparing it with the previous EU dispositions (Reg. (EC)\ud no. 104/2000 and no. 2065/2001). The exclusion of prepared and processed products and aquatic invertebrates\ud from the application of the mandatory seafood labelling provisions and the role of the mass\ud caterer operators with respect to the labelling requirements were identified as the two major shortcomings\ud that still need to be better addressed by the legislator. Overall, what emerged from this work is\ud that, if on the one hand the European legislation on seafood labelling has achieved important goals,\ud evolving and improving itself, on the other it is still controversial and plagued by the same problems as\ud 15 years ago. Therefore, the authors suggest that the regulation is modified at least extending its scope to\ud all products and to at all stages of the fishery logistic chai
Due to the social and legislative implications, the presence of Anisakis spp. larvae in fishery products has become a concern for both the consumers and the official Control Authorities. The issuance of a large number of provisions, aimed at better managing fish products intended to be consumed raw or almost raw and the associated risks, resulted in a very complicate legal framework. In this work, we analyzed the evolution of the normative through an overview on the local and international legislations, focusing on issues that are of practical interest for Food Business Operators (FBOs) in the fishery chain. In addition, we performed a survey across the Department of Prevention of the Italian Local Health Authorities (LHA) and the main fish markets in Italy to collect the operating procedures and the monitoring plans. Overall, we found many differences, due to the absence of a national reference standard for the management of the Anisakis risk. From this examination, it turns clear that only a participation of all the involved institutions, a strategy of synergistic interventions, as well as a correct training of FBOs, can result in an effective risk management and a proper risk communication, which should overcome states of confusion and unnecessary negative impacts on the economy.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.