ResumoAs partes aéreas da mandioca, representadas pelas folhas, hastes e caules, constituem-se como resíduos agroindustriais por serem desperdiçadas na colheita das raízes. Esse material possui valor protéico, vitaminas e mineiras, propiciando sua utilização como suplemento alimentar nas indústrias alimentícias. Alternativas neste sentido surgem para extrair a proteína das folhas e eliminar os agentes tóxicos e antinutricionais naturalmente presentes em sua composição. Neste contexto, o objetivo desta pesquisa foi avaliar rendimento, composição mineral e propriedades funcionais de concentrados protéicos, obtidos de folhas e parte aérea de mandioca. Na extração das proteínas, foram testados os métodos de (1) Precipitação Isoelétrica, (2) Fermentação Natural por 5 dias, (3) Fermentação por 48 horas e (4) Fermentação por 48 horas, seguida de ajuste de pH. Foi utilizado o delineamento fatorial 2x4, sendo os fatores o tipo de material (folhas e parte aérea) e o método de extração de proteína (quatro métodos), com três repetições. O Método 1 proporcionou os maiores rendimentos de concentrado protéico e extração de proteína das folhas de mandioca, porém, não foi verificada diferença significativa entre os métodos de extração para a parte aérea da mandioca. Os teores de Fe, Mn e Zn aumentaram nos concentrados protéicos da parte aérea da mandioca, com destaque para o Método 3. As capacidades de absorção de água e de óleo dos concentrados protéicos foram elevadas para os quatro métodos avaliados, indicando boa aplicação em produtos alimentícios. Palavras-chave: Proteína, métodos de extração, propriedades funcionais AbstractThe aerial parts of cassava, constituted by leaves, stalk sand stems, are considered as agro-industrial waste, so, they are thrown away during roots crop. This material has content of protein, vitamin and mineral; therefore, it can be used as a dietary supplement for food industries. Thus, alternatives have come to extract protein from leaves and remove toxic agent sand anti-nutrients that make part of their composition. In this context, this study aimed at evaluating yield, mineral composition and functional 1 Engenehira(s) Química(s), Mestre em Engenharia Agrícola,
Inflammatory Bowel Disease in Pediatric Patients With Cerebral PalsyT o the Editor: Cerebral palsy (CP) has not been associated with inflammatory bowel disease (IBD). We have diagnosed IBD in 5 children with CP in the last 2 years. We reviewed our pediatric patients seen from 1996 to 2009 at Children's Hospital, Colorado, with CP and/or IBD and identified 13 with CP and newly diagnosed IBD (6 Crohn disease, 5 ulcerative colitis, and 2 indeterminate colitis), providing an estimate of 1.8% (95% CI 0.97-3.08) for frequency of CP in IBD and an estimated cumulative incidence of 193 (95% CI 103-330) cases with IBD per 100,000 patients with CP.IBD patients with CP frequently presented with high white blood cell counts, often received infliximab or tacrolimus in the first month following diagnosis, and commonly developed perianal fistula during follow-up. Therefore, we suggest that CP is possibly associated with an aggressive form of IBD. We estimate a prevalence of CP in our patients with IBD (1.8%) that is higher than reported in the general population (0.36%) (1). The estimated cumulative incidence of IBD in our cross-sectional study of pediatric patients with CP was 193/100,000. Although we cannot explain this relation, potential mechanisms include causes of, or treatments for, CP, including special formula diets, that may affect the gastrointestinal microbiota or function (2); an undefined nutritional deficiency (3); and an altered intestinal environment in some children with CP that predisposes to immune dysregulation (4). The possible association between IBD and CP requires further evaluation in population-based studies.
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