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Our data are indicative for a novel regulatory role and underlying mechanism of LA in the negative regulation of a CSC-like phenotype in non-small cell lung cancer-derived cells.

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OBJECTIVE: Grammatophyllum speciosum is utilized to treat sore throats and bronchitis In Thai folk medicine. This study evaluated the in vitro activity and clinical efficacy of a G. speciosum pseudobulb decoction. METHODS: Measure of in vitro anti-ageing activity was performed using non-cell based assays as well as in CRL 2097 human fibroblast cells. A prophetic patch test method was used to determine skin irritation in 24 healthy Thai volunteers. A randomized double-blind, placebo-controlled trial was conducted with 24 subjects for 56 days after facial application to evaluate efficacy. The results were measured with Visioface â and Cutometer â MPA 580 as well as by visual observations. RESULTS: The total content of the antioxidant polyphenols in G. speciosum ethanolic extract (GSE) was 48.19 AE 0.39 mg EGCG equivalent per gram. The GSE possessed potent and higher antielastase activity more than EGCG. The extract was able to protect human fibroblasts against superoxide anion-induced cell death at the concentration of 10 µg mL À1 . In a clinical study, facial application of the serum containing 0.5% GSE was found to safely increase skin distensibility in healthy volunteers. Skin viscoelasticity and wrinkle volume were also significantly reduced (P < 0.05). CONCLUSION: Thus, both the in vitro and the clinical studies have illustrated the anti-wrinkle/anti-ageing benefits of GSE on human skin. 10 lg ml -1 . Dans une etude clinique, l'application sur le visage d'un s erum contenant 0,5 % du GSE a permis d'augmenter la distensibilit e de la peau chez des volontaires sains en toute s ecurit e. La visco elasticit e de la peau et le volume des rides ont egalement et e significativement r eduits (P < 0.05). CONCLUSION: Ainsi, les etudes in vitro tout comme les etudes cliniques ont illustr e les b en efices antirides/anti-âge du GSE sur la peau humaine.

BackgroundEpithelial to mesenchymal transition (EMT) has been shown to be a crucial enhancing mechanism in the process of cancer metastasis, as it increases cancer cell capabilities to migrate, invade and survive in circulating systems. This study aimed to investigate the effect of essential element zinc on EMT characteristics in lung cancer cells.MethodsThe effect of zinc on EMT was evaluated by determining the EMT behaviors using migration, invasion and colony formation assay. EMT markers were examined by western blot analysis. Reactive oxygen species (ROS) were detected by specific fluorescence dyes and flow cytometry. All results were analyzed by ANOVA, followed by individual comparisons with post hoc test.ResultsThe present study has revealed for the first time that the zinc could induce EMT and related metastatic behaviors in lung cancer cells. Results showed that treatment of the cells with zinc resulted in the significant increase of EMT markers N-cadherin, vimentin, snail and slug and decrease of E-cadherin proteins. Zinc-treated cells exhibited the mesenchymal-like morphology and increased cancer cell motility with significant increase of activated FAK, Rac1, and RhoA. Also, tumorigenic abilities of lung cancer cells could be enhanced by zinc. Importantly, the underlying mechanism was found to be caused by the ability of zinc to generate intracellular superoxide anion. Zinc was shown to induce cellular superoxide anion generation and the up-regulation of EMT markers and the induced cell migration and invasion in zinc-treated cells could be attenuated by the treatment of MnTBAP, a specific superoxide anion inhibitor.ConclusionKnowledge gains from this study may highlight the roles of this important element in the regulation of EMT and cancer metastasis and fulfill the understanding in the area of cancer cell biology.