Matsutake mushroom
Mycorrhizal isolationNuclear ribosomal RNA gene (rDNA) IGS1 region a b s t r a c t Tricholoma matsutake is one of the most highly valued ectomycorrhizal mushrooms, primarily associated with conifers. Here, we examined the association of T. matsutake with hemlock and fir species native to the subalpine forests of Japan. Basidiomata of T. matsutake were harvested from the forests of Honshu Island, Japan, along with two soil samples directly beneath the basidiomata; ectomycorrhizal root tips were also collected, and used for fungal isolation and molecular analyses. Mycorrhizal fungi were isolated from the roots of Abies veitchii (Veitch's fir) and Tsuga diversifolia (northern Japanese hemlock). These fungi were identified as T. matsutake based upon their production of whitish mycelial colonies on nutrient agar media, and confirmed using molecular analyses; the ectomycorrhizal association between T. matsutake and A. veitchii was also confirmed by mycorrhizal synthesis in vitro. This work represents the first description of T. matsutake ectomycorrhizations withAbies and Tsuga species, as well as a detailed morphological description of these associations. /locate /m yc m y c o s c i e n c e 5 6 ( 2 0 1 5 ) 4 0 2 e4 1 2 http://dx.
Abstract. Suwannasai N, Dokmai P, Yamada A, Watling R, Phosri C. 2020. First ectomycorrhizal syntheses between Astraeus sirindhorniae and Dipterocarpus alatus (Dipterocarpaceae), pure culture characteristics, and molecular detection. Biodiversitas 21: 231-238. This study provides the first mycorrhization of Astraeus sirindhorniae and its cultural characteristics on nutrient media. An attempt has been made to introduce spore suspension and mycelial inocula of A. sirindhorniae onto seedlings of Dipterocarpus alatus. After 6 months seedlings were harvested, measured for growth and morphological descriptions of the ectomycorrhizas formed with D. alatus seedlings were made. The fungus has increased the growth of D. alatus seedlings. Further, it can be confirmed that the primers designed (GAPK126F/GAPK379R) have been successful when applied for the detection of ectomycorrhizal formation of A. sirindhorniae in vivo.
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