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Application of assembly methods for personal genome analysis from next generation sequencing data has been limited by the requirement for an expensive supercomputer hardware or long computation times when using ordinary resources. We describe CompStor™ Novos, achieving supercomputer-class performance in de novo assembly computation time on standard server hardware, based on a tieredmemory implementation. Run on commercial off-the-shelf servers, Novos assembly is more precise and 10-20 times faster than that of existing assembly algorithms. Furthermore, we integrated Novos into a variant calling pipeline and demonstrate that both compute times and precision of calling point variants and indels compare well with standard alignment-based pipelines. Additionally, assembly eliminates bias in the estimation of allele frequency for indels and naturally enables discovery of breakpoints for structural variants with base pair resolution. Thus, Novos bridges the gap between alignment-based and assemblybased genome analyses. Extension and adaption of its underlying algorithm will help quickly and fully harvest information in sequencing reads for personal genome reconstruction.

Chiral Synthons from Carvone. Part 35. Carvone Based Approaches to Chiral Functionalized B-seco-Taxanes. -Starting from carvone (I) the synthesis of the B-seco-taxane derivative (VIII) is achieved. This method is extended incorporating an oxygen functionality in the taxane skeleton. Phenylacetylene (IX) is used as a 1,2-dioxygenated ethyl group equivalent. Beside the synthesis of C-aromatic bis-nortaxane derivatives, a C-20 nortaxane derivative is synthesized employing an appropriate aliphatic C-ring precursor. -(SRIKRISHNA, A.; REDDY, T. J.; KUMAR, P. P.; J.

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