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AH Plus appears to bond to the organic phase of dentine. This bond influences its sealing ability.DOI: https://doi.org/10.1111/j. 1365-2591.2010.01848.x Posted at the Zurich Open Repository and Archive, University of Zurich ZORA URL: https://doi.org/10.5167/uzh-50248 Accepted Version Originally published at: Neelakantan, P; Subbarao, C; Subbarao, C V; De-Deus, G; Zehnder, Matthias (2011). The impact of root dentine conditioning on sealing ability and push-out bond strength of an epoxy resin root canal sealer. International Endodontic Journal, 44 (6) AbstractAim To investigate the impact of dentine conditioning on sealing ability and dentine bond strength of an epoxy resin sealer.Methodology Root canals in 90 single-rooted teeth were instrumented using a rotary Ni-Ti system. Fifty canals were irrigated with water during instrumentation, 40 with 3% NaOCl. A final flush was performed in the water-irrigated specimens with water (negative control), 3% NaOCl, 17% EDTA, 7% maleic acid (MA), or 2% chlorhexidine. The hypochlorite irrigated specimens received a final flush with a decalcifying agent (EDTA or MA) and then 3% NaOCl or 3% NaOCl and then the decalcifying agent (n = 10, each). Canals were all filled with AH Plus. Fluid transport was measured on day 3 and 30. Roots were then sectioned, and push-out tests were performed in coronal, middle, and apical root thirds. Results were analyzed using analysis of variance (ANOVA) with Bonferroni's adjustment. Spearman's rank correlation was computed between fluid transport and push-out bond strength.Results Leakage decreased over time (P < 0.05). Push-out bond strength was highest in coronal and lowest in apical root thirds. Irrigating protocols with final application of a decalcifying agent resulted in a fold decrease in leakage and increase push-out bond strength values. If NaOCl was applied last, the effect of the decalcifying agent was abolished.Chlorhexidine had no impact on the outcomes. Fluid transport and push-out bond strength correlated strongly (rho = -0-83).Conclusions AH Plus appears to bond to the organic phase of dentine. This bond influences its sealing ability.2

Neelakantan P, Varughese AA, Sharma S, Subbarao CV, Zehnder M, De‐Deus G. Continuous chelation irrigation improves the adhesion of epoxy resin‐based root canal sealer to root dentine. International Endodontic Journal Abstract Aim  To test the impact of continuous chelation by NaOCl+ etidronic acid (HEBP) during instrumentation, and a final rinse of EDTA or NaOCl + HEBP on the dentine bond strength of an epoxy resin sealer (AH Plus). Methodology  Single‐rooted teeth (n = 100) were divided into five groups (n = 20) based on the irrigation protocol and their root canals instrumented using a rotary Ni‐Ti system: 2.5% NaOCl during instrumentation followed by bi‐distilled water (G1) or 17% EDTA (G2) as final rinse; 1 : 1 mixture of 5% NaOCl and 18% HEBP during instrumentation, and the same mixture (G3), 17% EDTA (G4) or bi‐distilled water (G5) as final rinse. Canals were filled with AH Plus. Roots were sectioned, and push‐out tests were performed in coronal, middle and apical root thirds. Results were analysed using analysis of variance (anova) and Bonferroni test for multiple comparisons. The alpha‐type error was set at 0.05 for all the analyses. Results  Push‐out bond strength was highest in coronal and lowest in apical root thirds (P < 0.05). Groups that used NaOCl + HEBP irrigation during instrumentation had significantly higher bond strengths than groups following the NaOCl‐EDTA irrigation in all root thirds (P < 0.05). The use of a strong chelator as final flush further increased bond strengths (G4, P < 0.05). Conclusion  The continuous chelation irrigation protocol optimizes the bond strength of an epoxy resin sealer to dentine.