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AH Plus appears to bond to the organic phase of dentine. This bond influences its sealing ability.DOI: https://doi.org/10.1111/j. 1365-2591.2010.01848.x Posted at the Zurich Open Repository and Archive, University of Zurich ZORA URL: https://doi.org/10.5167/uzh-50248 Accepted Version Originally published at: Neelakantan, P; Subbarao, C; Subbarao, C V; De-Deus, G; Zehnder, Matthias (2011). The impact of root dentine conditioning on sealing ability and push-out bond strength of an epoxy resin root canal sealer. International Endodontic Journal, 44 (6) AbstractAim To investigate the impact of dentine conditioning on sealing ability and dentine bond strength of an epoxy resin sealer.Methodology Root canals in 90 single-rooted teeth were instrumented using a rotary Ni-Ti system. Fifty canals were irrigated with water during instrumentation, 40 with 3% NaOCl. A final flush was performed in the water-irrigated specimens with water (negative control), 3% NaOCl, 17% EDTA, 7% maleic acid (MA), or 2% chlorhexidine. The hypochlorite irrigated specimens received a final flush with a decalcifying agent (EDTA or MA) and then 3% NaOCl or 3% NaOCl and then the decalcifying agent (n = 10, each). Canals were all filled with AH Plus. Fluid transport was measured on day 3 and 30. Roots were then sectioned, and push-out tests were performed in coronal, middle, and apical root thirds. Results were analyzed using analysis of variance (ANOVA) with Bonferroni's adjustment. Spearman's rank correlation was computed between fluid transport and push-out bond strength.Results Leakage decreased over time (P < 0.05). Push-out bond strength was highest in coronal and lowest in apical root thirds. Irrigating protocols with final application of a decalcifying agent resulted in a fold decrease in leakage and increase push-out bond strength values. If NaOCl was applied last, the effect of the decalcifying agent was abolished.Chlorhexidine had no impact on the outcomes. Fluid transport and push-out bond strength correlated strongly (rho = -0-83).Conclusions AH Plus appears to bond to the organic phase of dentine. This bond influences its sealing ability.2

The use of NaOCl after or in combination with a chelator caused the greatest reduction of E. faecalis. Diode laser and Er:YAG laser activation were superior to ultrasonics in dentinal tubule disinfection.