Mating elicits two well-defined reactions in sexually matured females of many insects: reduction of receptivity and increased oviposition. These post-mating responses have been shown to be induced by factors synthesized in the reproductive tract of the adult male and transferred in the seminal fluid to the female during copulation. One of these factors, named sex-peptide (SP), has been identified in Drosophila melanogaster. Using an in vitro radiochemical assay, we show that synthetic sex-peptide considerably activates juvenile hormone III-bisepoxide (JHB3) synthesis in corpus allatum (CA) excised from Days 3 and 4 post-eclosion virgin females. Base levels are significantly lower at emergence (Day 0) than on subsequent days, and only weak stimulation is obtained on Day 1, while none is obtained on Day 2, where maximal basal synthesis occurs. The CA of mated females cannot be stimulated further for at least 7 days, but regain responsiveness by Day 10 after mating. Synthesis of JHB3 stimulated by SP in vitro persists for at least 4 h after removal of the peptide. Development of responsiveness of the CA to SP in vitro is compared with development of the post-mating reactions of sex-peptide injected virgin females. Our results suggest that the CA is a direct target for SP in vivo and that sexual maturity is established separately for the two post-mating reactions.
Pyriproxyfen, a juvenile hormone (JH) mimic, is a biorational insecticide that disrupts insect development. It is one of the principal insecticides being used to control Bemisia tabaci (Gennadius) on cotton, and has many environmentally positive attributes that make it compatible with integrated pest management (IPM) programs. In Israel, a high level of resistance to pyriproxyfen has been observed in several isolated regions. Here, tests were conducted to establish whether temporal refuges from exposure to pyriproxyfen could be useful for restoring the effectiveness of the compound. Resistance was found to decrease by a factor of 8 when exposure to pyriproxyfen was ceased for 13 generations. Reversal of resistance was accompanied with increased biotic fitness of the revertant colony. By incorporating experimental estimates of nymph survival, sex ratio, fecundity, egg hatching rate and developmental time, the seasonal cost per generation for resistant insects was estimated to be 25%. A genetic simulation model, optimized by empirical data from bioassays, predicted fitness cost per generation of 19% for resistant homozygous (RR) females and hemizygous (R) males, and produced rates of reversal similar to the experimental results. The model also predicted that, even after 5 years ( approximately 55 generations) without pyriproxyfen treatments, the frequency of the resistance allele (R) will still remain high (0.02). It is therefore concluded, on the basis of experimental and modeling results, that the effectiveness of temporal refuges for reversing development of resistance to pyriproxyfen in B. tabaci may be limited.
Generalist insect can utilize two different modes for regulating their detoxification genes, the constitutive mode and the induced mode. Here, we used the Bemisia tabaci sibling species MEAM1 and MED, as a model system for studying constitutive and induced detoxification resistance and their associated tradeoffs. B. tabaci adults were allowed to feed through membranes for 24 h on diet containing only sucrose or sucrose with various phytotoxins. Quantitative real-time PCR analyses of 18 detoxification genes, indicated that relatively few transcripts were changed in both the MEAM1 and MED species, in response to the addition of phytotoxins to the diet. Induced transcription of detoxification genes only in the MED species, in response to the presence of indole-3-carbinol in the insect’s diet, was correlated with maintenance of reproductive performance in comparison to significant reduction in performance of the MEAM1 species. Three genes, COE2, CYP6-like 5 and BtGST2, responded to more than one compound and were highly transcribed in the insect gut. Furthermore, functional assays showed that the BtGST2 gene encodes a protein capable of interacting with both flavonoids and glucosinolates. In conclusion, several detoxification genes were identified that could potentially be involved in the adaptation of B. tabaci to its host plants.
Adult female Drosophila melanogaster corpus allatum (CA) synthesize JHB3 from endogenous and exogenous precursors in vitro. We present evidence supporting the thesis that biosynthesis proceeds from precursor FA via initial epoxidation and terminal methylation on the basis of the following: (1) Methyl farnesoate is not epoxidized to JHIII or JHB3; (2) Authentic JHIII is not epoxidized to JHB3; and (3) FABE is markedly metabolized to JHB3. Cerebral allatostatic factors act at some stage subsequent to FA and this precursor is not normally rate-limiting. Additionally, neural inhibition from the brain acts at some biosynthetic step prior to FA.
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